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UltraRIPA Buffer

Product#: FNK-F015B

$263.12

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UltraRIPA Buffer

Cat. No.: FNK-F015B

Size: 100mL

Storage: 4°C

Description:

Next-generation RIPA buffer that greatly improves the extraction efficiency of membrane proteins. Because proteins can be solubilized under non-denaturing conditions, it can also be used in experiments such as enzyme activity and immunoprecipitation.

Although RIPA (Radio-ImmunoPrecipitation Assay) buffer shows little effects on denaturation of proteins unlike 1-2% SDS buffer, it has high protein extraction ability from tissues and cells. The proteins extracted with RIPA buffer generally keep the functions such as protein structures and enzymatic activity. Therefore, RIPA buffer is one of the most useful buffer to extract proteins from tissues and cells for various assays of functional proteins.

However, there are fractions even RIPA buffer cannot solubilize. Those fractions are called DRM (Detergent resistant membrane) or lipid raft. In general, it is difficult to analyze functions of the proteins because of its hard solubility in mild extraction buffers including RIPA buffer. UltraRIPATM buffer enable us to more efficiently extract DRM proteins than RIPA buffer, with little effects of denaturation. The proteins extracted with UltraRIPATM buffer is useful for enzymatic activity and immunoprecipitation etc.

Specifications

Format: 1x UltraRIPATM Buffer, Ready to use

Product Shelf Life: 1 year after the date of receipt

For Research Use Only.

Protein Extraction

Depiction of mouse brain lysed with RIPA buffer and homogenized by a dounce tissue grinder and sonicator.

Depiction of mouse brain lysed with RIPA buffer and homogenized by a dounce tissue grinder and sonicator.

A mouse brain was lysed with RIPA buffer and homogenized by a dounce tissue grinder and sonicator. After centrifugation of the lysate to remove RIPA-soluble supernatant, 2% SDS buffer, RIPA buffer, or UltraRIPATM buffer was added to the RIPA-insoluble pellet and resuspended well. Each supernatant after centrifugation was applied to silver stain and BCA protein assay.

Experimental result:

Comparison of UltraRIPA buffer vs other industry alternatives.

Comparison of UltraRIPA buffer vs other industry alternatives.

More than 70% of protein was extracted from RIPA-insoluble fraction using UltraRIPA^TM buffer.

Enzymatic activity:

Experimental procedure

Comparison of CHO cells directly lysed in 1% Triton X-100 containing buffer, RIPA buffer, UltraRIPATM buffer, or 2% SDS buffer, respectively.

Comparison of CHO cells directly lysed in 1% Triton X-100 containing buffer, RIPA buffer, UltraRIPATM buffer, or 2% SDS buffer, respectively.

Experimental result

LDH activity in supernatant extracted with UltraRIPATM buffer was equivalent to those in Triton X-100 containing buffer and RIPA buffer.

Reference

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