HRP stabilizer
Cat. No.: FNK-BCL-HSS-01
Size: 50ml
Storage: 4℃ (1 year)
Product outline:
HRP stabilizer is the storage solution for HRP-labeled antibody for various immuno-assays such as ELISA and Western blot. The solution can be used not only for storage of stock solution at high concentration or working solution at working range concentration for more than 1 year.
Features:
1. Stock solution of that HRP-labeled antibody at high concentration can be stored.
2. Working solution of HRP-labeled antibody (a few 10 ng to a few 10 μg/mL ) that can be stored.
3. Easy to use (just dilute or dissolve HRP-labeled antibody)
How to use
1. Preparation of stock solution at high concentration
When antibodies are labeled with an HRP-labeling kit, concentrate the labeled antibody using an ultrafiltration membrane, and dilute it with this product.
In order to maintain the concentration of the active ingredient in this product, dilute the labeled-antibody at least 10 times. Store the diluted antibody at 2 - 8°C avoiding from light. When storing a ready-made HRP-labeled antibody, dilute it with this product at least 10 times as above. If necessary, concentrate the antibody solution to the appropriate concentration using an ultrafiltration membrane before diluting.
2. Preparation of antibody solution at working range concentration
Dilute the stock solution prepared in step 1. (above) with this product to an appropriate concentration (e.g., several tens of ng to several tens of µg/mL).
Store the diluted solutions at 2 - 8°C avoiding from light. When diluting antibody solution not prepared with this product, dilute it with this product at least 10 times.
<Note> The stability of HRP-labeled antibodies is affected by its purity. Please use antibodies highly purified by affinity chromatography, etc.
Estimated storage period for labeled antibodies
When the labeled antibody was stored at a high concentration (0.5 mg/mL), its activity remained unchanged for over 1 year under both 4°C and 25°C (accelerated test) conditions. If stored properly, labeled antibodies seems to be stable for several year under refrigerated conditions.
When the labeled antibody was stored at a low concentration (50ng/mL), its activity remained unchanged for more than 3 months at 25°C (accelerated test). Even at concentrations as low as several tens of ng/mL, labeled antibodies seems to be stable for more than a year under refrigeration.
<Note> Stability of HRP-labeled antibodies is affected by the purity and nature of the labeled antibodies. If necessary, evaluate the activity of the labeled antibodies during the storage period.
