Crystal Violet Staining Solution (Water-Based)
Cat No.: DCP-CVW1X
Size: 500 mL
Sterile, Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Crystal Violet Staining Solution delivers 1% crystal violet in ultrapure water (Type I, 18.2 MΩ·cm), designed for assays requiring zero alcohol interference.
Key Features:
- Zero Alcohol: Eliminates solvent-induced cell shrinkage (≤2% dimensional change vs. 15% in ethanol)
- Flexible Fixation: Compatible with methanol, formaldehyde, or cryopreservation methods.
- Electrophoresis Ready: 0.04 μm filtered for nucleic acid counterion staining.
Applications:
- Stem cell colony counting (CFU assays)
- Live/dead discrimination in microfluidic devices
- DNA band visualization (0.1 μg/mm2 sensitivity)
- Classroom demonstrations (ANSI Z358.1 eyewash compatible)
Composition:
| Ingredients |
Concentration |
| Crystal Violet |
1% |
| Water |
99% |
Quality Control:
- Appearance: Purple solution
- pH: 6.8-7.2
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Store at 2-8°C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.