Long ssDNA Gel Extraction Kit

Description

The Long ssDNA Gel Extraction Kit is a highly-specialized kit for long ssDNA purification from agarose gel. The spin column, buffer formulations and purification protocol are optimized for high recovery yields and high purity of long ssDNA. Long ssDNA purified with the kit is
high-quality, with low mechanical degradation and without ultraviolet light-induced damage from the purification process. The purified long ssDNA is suitable for molecular biology and biotechnology applications.
The Long ssDNA Preparation Kit can be universally used for purification and clean-up of long ssDNA.

Features and Specifications:

• Optimized for long ssDNA.
• High recovery yield (typically 55-75%).
• High purity.
• Low mechanical degradation.
• No UV light-induced DNA damage.
• Direct monitoring of the migration of long ssDNA blue bands in the gel during electrophoresis.
• Excision of long ssDNA under ambient light.
• Guanidine thiocyanate is used as chaotropic salts. NaI is not used.*
• ssDNA size for excision: 3,000 - 10,000 bases(10kb kit).
• ssDNA size for excision: 500 - 3,000 bases(3kb kit)
• Binding capacity on a Spin Column for ssDNA binding is up to 10μg.
• Elution volume: ≥ 15 μl.
  • Residual NaI may be difficult to remove, and reduces the efficiency of downstream enzymatic reactions.