Tris-HCl Buffer, Sterile [1.5X]
Cat No.: DCP-THB1.5X
Size: 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Tris-HCl buffer is widely used in molecular biology techniques like gel electrophoresis, nucleic acid work, protein extraction and purification.It can be used as resolving gel buffer when casting polyacrylamide gels for protein electrophoresis. It fully deprotonates glycine from the running buffer, allowing proteins to separate based on size. It provides the optimal ionic environment for protein migration and separation.
Advantages:
- Optimal pH for protein separation: The alkaline pH of 8.8 creates an ideal environment for protein separation in the resolving gel.
- Enhances resolution: The buffer helps create sharp protein bands and improves overall resolution of separated proteins.
- Part of a discontinuous buffer system: When used with stacking gel buffer (pH 6.8) and running buffer (pH 8.3), it forms a discontinuous system that concentrates proteins before separation.
- Versatility: It's compatible with both SDS-PAGE and native PAGE protocols.
- Stability: The high pH contributes to the stability of the gel structure during electrophoresis.
- Convenience: Available as a ready-to-use solution, saving time in gel preparation.
Application:
- SDS-PAGE: Used in the preparation of resolving gels for denaturing protein electrophoresis.
- Native PAGE: Can be used for non-denaturing protein separation when SDS is omitted.
- Protein molecular weight determination: Helps create a gel matrix suitable for separating proteins based on their molecular weights.
- Protein complex analysis: When used in Tris-glycine systems, it's effective for separating a broad range of molecular weight proteins.
- Quality control: Used in various protein analysis applications in research and industrial settings.
- Preparative electrophoresis: Useful for isolating specific proteins from complex mixtures for further analysis or purification.
- 2D gel electrophoresis: Often used in the second dimension of 2D gel electrophoresis for high-resolution protein separation.
Usage:
When casting polyacrylamide gels:
- Mix the Resolving Gel Buffer with acrylamide solution, water, and other components according to the desired gel percentage.
- Add polymerization initiators (APS and TEMED) just before pouring the gel.
- Pour the resolving gel mixture into the casting apparatus, leaving space at the top for the stacking gel.
- Allow the resolving gel to polymerize before adding the stacking gel on top.
Composition:
- 1.5M Tris base
- HCl to adjust the pH
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 8.8
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Sterility: Filtered 0.1 micron Twice + 0.04 micron in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
