Anti-Rad51(S.pombe) Antibody, rabbit serum
Product#: FNK-63-001
$346.79
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Ships in 1-2 Weeks
Anti-Rhp51 / Rad51(S. pombe) Antibody, rabbit polyclonal
General information
| Cat. No. | ：FNK-63-001 |
| Size | ：100 uL |
| Host Species | ：Rabbit |
| Immunogen | ：Purified recombinant full-length Rhp51 protein |
| Application |
|
| Reactivity | ：Schizosaccharomyces pombe. Specificity has been validated with rhp51 mutant for WB (Fig.1) |
| Storage | ：Sent at 4℃ and store at -20℃. |
| Form | ：Rabbit antiserum added with 0.09 % sodium azide |
| Data Link | ：UniProtKB/Swiss-Prot P36601 (RAD51_SCHPO) |
Description
Rhp51 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of E.coli RecA protein and Rad51 proteins of eukaryotes, which play a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands.
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Fig.1 Western blot analysis of Rhp51 in the whole cell extracts. M: Molecular size markers (kD) |
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Fig. 2 Rhp51 foci formation observed after DNA damage:
S. pombe cells without or with MMS (0.025%) treatment for 1 h were processed for indirect immunofluorescence staining with anti-Rhp51 antibody (1/500 dilution). The Rhp51 foci formation is induced by DNA-damage treatment with MMS. |
References: This antibody has been used in the following publications.
- Akamatsu Y et al. Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15770-5. WB, IP (S. pombe)
- Kibe T et al. Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer. Nucleic Acids Res. 2003 Sep 1;31(17):5054-63. ChIP (S. pombe)
- Lambert S et al “Gross chromosomal rearrangements and elevated recombination at an inducible site-specific replication fork barrier” Cell 121: 689-702 (2005) PMID: 15935756 IF (S. pombe)
- Morishita T et al ” Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediates” Mol Cell Biol 25: 8074-8083 (2005) PMID: 16135799 IF (S.pombe)
- Haruta N et al "The Swi5-Sfr1 complex stimulates Rhp51/Rad51-and Dmc1-mediated DNA strand exchange in vitro”Nat Struc Mol Biol 13: 823-830 (2006) PMID: 16921379 WB, IP (S. pombe)
