1. Good lineality against enzyme volume
2. Equal signal intensity as widely used high signal TMB solution
3. High cost performance
4. Good storage stability (1 year at 4°C)

How to Use

1. Prepare microplates so that chromogenic reaction is ready to start.

2. Remove the TMB solution bottles from refrigerator; transfer half of required amount into reservoir from Solution A bottle, other half from Solution B bottle, and mix well. Please return TMB solution bottles to the refrigerator as soon as possible.

3.  From the reservoir, add 100μL of TMB solution into wells of microplate by using 8-channel pipette, and let it stay for 30 min in light-tight container at room temperature. Blue colors appear during this stage.

4. After incubation, stop the reaction by adding 50μL of 2M H2SO4. The color changes from blue to yellow. Read absorbance at 450nmby plate reader.