TDzyme® TL Pack
Cat. No. TDP0010
Description
TDzyme® is a tissue dissociation enzyme produced from E. coli by using the manufacturing method of genetic recombination.
TDzyme® is manufactured by genetic recombination of collagenase derived from Clostridium histolyticum.
TDzyme® is manufactured in animal source free condition, so there is no risk of contamination with viruses.
TDzyme® is prepared by the purification of each enzyme with high purity and then mixing them by using column chromatography, which lead to high quality and lot-to-lot consistency.
TDzyme® is suitable for tissue dissociation and cell culture due to low endotoxin specification.
TDzyme® is manufactured in lyophilized form, which secures enhanced stability in storage.
TDzyme® is contained in a smart device enabling simultaneous dissolution and in-line filtration of lyophilized enzyme, which provides easy handling and contributes to reducing any contamination risks.
TDzyme® is manufactured by genetic recombination of collagenase derived from Clostridium histolyticum.
TDzyme® is manufactured in animal source free condition, so there is no risk of contamination with viruses.
TDzyme® is prepared by the purification of each enzyme with high purity and then mixing them by using column chromatography, which lead to high quality and lot-to-lot consistency.
TDzyme® is suitable for tissue dissociation and cell culture due to low endotoxin specification.
TDzyme® is manufactured in lyophilized form, which secures enhanced stability in storage.
TDzyme® is contained in a smart device enabling simultaneous dissolution and in-line filtration of lyophilized enzyme, which provides easy handling and contributes to reducing any contamination risks.
Application
TDzyme® consists of TDzyme C and TDzyme T.
- TDzyme C is collagenase formulated to contain minimal amount of thermolysin. It is suitable for breakdown of various tissues including pancreatic islets.
- TDzyme T is thermolysin and can be mixed with TDzyme C to dissociate stronger tissues such as adipose tissues, cartilage, and extracellular matrices of skin and blood vessels.
Storage and Stability
- Please store the eluted enzyme solution immediately on ice.
- Freeze unused enzyme solution as needed.
- Please keep in mind that repeated freezing and thawing of the enzyme solution may result in decreased enzyme activity.
Recombinant tissue dissociation enzyme
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Genetic engineering is used to secure animal source free condition. |
| - Collagenase class I (collagenase G) originating from Clostridium histolyticum - Collagenase class II (collagenase H) originating from Clostridium histolyticum - Thermolysin originating from Geobacillus stearothermophilus |
Purified & blended type enzyme (not crude type)
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- Highly purified using column chromatography technology - Blended with three enzymes at fixed ratio - High lot-to-lot consistency - High purity & low endotoxin |
Application on target tissues
| Tissue Type | TDzyme TL Pack | TDzyme TM Pack |
| Adipose | √ | |
| Cornea | √ | |
| Heart | √ | |
| Kidney | √ | |
| Liver | √ | |
| Lung | √ | √ |
| Lymph Node | √ | √ |
| Ovarian | √ | |
| Pancrease (islets) | √ | |
| Skin | ||
| Spleen | √ |
Innovative packaging technology
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Ordering information
| Product name | Product No. | Contents | |
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TDzyme® TL Pack | TDP0010 | TDzyme® C (5mg), 2ea |
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TDzyme® TM Pack | TDP0008 | TDzyme® C (5mg) + TDzyme® T (3mg) |
- TDzyme® C (blue cap): Collagenase (including minimal amount of thermolysin)
- TDzyme® T (red cap): Thermolysin
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