Borate Buffered Saline [1X]
Cat No.: DCP-BBS1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Borate Buffered Saline (BBS) is a commonly used buffer in biochemical and molecular biology applications. It is a versatile buffer with alkaline pH, isotonicity, and bactericidal properties and can emulate physiological conditions. It can be used as an isotonic solution for biological applications. It can be used to dilute substances in biochemical and molecular biology protocols. BBS has strong bactericidal properties, it determines if heat-killed, borate-buffered suspensions of Gordonia bronchialis, Rhodococcus coprophilus or Tsukamurella inchonensis can treat canine flea allergy. Additionally, it is used to investigate the effects of lentivirus-mediated siRNA targeting human metastasis suppressor 1 gene on the invasive and metastatic potentials of hepatocellular carcinoma.
Advantages:
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Borate-buffered saline exhibits strong bactericidal effects, which can be advantageous in biochemical techniques where preventing bacterial growth is desirable.
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It has a similar osmotic pressure as biological fluids, so it can be used for applications involving biological samples or systems that require physiological conditions.
Applications:
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Wash and blocking buffer by adding Tween 20 and milk powder.
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Coating microtiter plates or other surfaces with proteins or antibodies in ELISA.
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Diluent for biological samples while maintaining a stable pH environment.
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With bactericidal properties, it is used in biochemical applications where preventing bacterial growth is desirable.
Composition:
| Ingredients |
Concentration |
| Boric Acid |
1 M |
| Sodium Hydroxide |
0.25 M |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 8.5
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Sterility: Filtered 0.1 micron Twice in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
