Anti-Rad18 (human) antibody, rabbit polyclonal IgG
Product#: FNK-70-023
$346.79
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Anti-Rad18 (human) antibody, rabbit polyclonal IgG
General information
| Cat. No. | ：FNK-70-023 |
| Size | ：100 ug |
| Antigen | ：Human |
| Host Species | ：Rabbit |
| Reactivity | ：Human Rad18. Not reactive to mouse Rad18 |
| Immunogen | ：GST-fusion protein containing 113 carboxyl terminal of human Rad18 |
| Antibody | ：Polyclonal rabbit IgG fraction pre-adsorbed with GST affinity column 1mg/ml in PBS, 50% glycerol, 0.09% sodium azide |
| Application | ：1) Western blotting (1,000 fold dilution) ：2) Indirect immunofluorescence staining ：3) Immunoprecipitation |
| Storage | ：-20℃ |
| Form | ：1 mg/ml PBS (20mM Na-phosphate pH7.5 , 0.15M NaCl), 50% glycerol |
| Data Link | ：UniProtKB/TrEMBL C9J0Q4 (C9J0Q4_HUMAN) |
Description
The Rad6-Rad18 pair of genes plays a critical role in post-replication repair of damaged DNA. Rad6 protein functions as an E2 enzyme and Rad18 as an ubiquitine ligase (E3) which ubiquitinates PCNA. Rad18 recruits translesion DNA polymerases to damaged DNA (ref 1~3).
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| Fig.1 Indirect immunofluorescence staining of Rad18 protein in GM637 cells. Rad18 protein is stained as yellow dots in nuclei. |
Fig.2 Identification of Rad18 protein in crude extract of A549 cells by Western blotting.
The lower thick band is native Rad18 and the upper thin band is mono-ubiquitinated Rad18 protein (ref. 1)
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References
- Miyase S et al “Differential regulation of Rad18 through Rad6-dependent mono- and polyubiquitination” J Biol Chem 280: 515-524 (2005) PMID: 15509568
- Tateishi S et al “Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens” PNAS 97: 7927-7932 (2000) PMID: 10884424
- Watanabe K et al “Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination” EMBO J 23: 3886-3896 (2004) PMID: 15359278
Aliases for RAD18 Gene
- RAD18 E3 Ubiquitin Protein Ligase 2 3 5
- RNF73 2 3 4
- RING-Type E3 Ubiquitin Transferase RAD18 3 4
- Postreplication Repair Protein RAD18 3 4
- E3 Ubiquitin-Protein Ligase RAD18 3 4
- RING Finger Protein 73 3 4
- HRAD18 3 4
- HHR18 3 4
- Postreplication Repair Protein HRAD18p 3
- RAD18 Homolog (S. Cerevisiae) 2
- RAD18, S. Cerevisiae, Homolog 3
- RAD18 Homolog 3
- EC 2.3.2.27 4
- RAD18 5
