YB Rapid Ligation Kit

Cat. No.:  YBB-FYC003-100R


Description

Yeastern’s yT4 DNA ligase catalyzes the joining of two strands of DNA between the 5′ – phosphate and the 3′ – hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. The enzyme also repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids. YB Rapid Ligation Kit is designed for efficient ligation of DNA inserts into vectors in just 5 minutes.

 
Features

• Either sticky- or blunt-end, ligation can be achieved within 5 minutes
• The reaction mixture can be used directly for bacterial transformation

Applications

• Join double-strand DNA with cohesive or blunt termini
• Join oligonucleotide linkers to DNA sequence with the blunt end
• Repair nicks in duplex DNA, RNA, or DNA-RNA hybrids

Combo Products


Quality Control

 Undetectable endo-deoxyribonuclease and exo-deoxyribonuclease activities; Undetectable ribonuclease activity; Undetectable degradation of labeled oligonucleotide; Passed the test of the capability to join cohesive- and blunt-ended DNA fragments.

Specification

 Store at  - 20°C 

 

# YBB-FYC003-100R (100 rxns)

yT4 DNA Ligase	100 μl (3 U/μl)
10× Ligation Buffer A	200 μl
10× Ligation Buffer B	200 μl

Storage Buffer

Tris-HCl (pH7.5)	20 mM
KCl	50 mM
DTT	1 mM
EDTA	0.1 mM
Glycerol stabilizers	50%

10× Ligation Buffer A
 

Tris-HCl	0.4M
MgCl2	0.1 mM
DTT	0.1 mM
EDTA	5 mM
ATP (pH5.0 at 25°C)	5 mM
The performance of this buffer depends on the integrity of ATP. Store the buffer in small aliquots at -20°C to minimize degradation of ATP and DTT

10× Ligation Buffer B

The buffer contains an enhancer which dramatically increases ligation efficiency for blunt end DNA