Reverse Transcriptase

Image	Cat.No:	Size	Storage	Link
	CSB-DEM025	10000U	Long term storage at -20 °C, short term storage at 4 °C
	SB-G3415-10KU	10KU	Transport in wet ice packs; store at -20°C, valid for 12 months.
	SB-G3416-10KU

Precautions for SB-G3415-10KU & SB-G3416-10KU
 
1. RNA is easy to be degraded, standardize the operation to avoid RNase contamination;
2. The reverse transcription product can be stored at -20°C for short-term storage. If it needs to be stored for a long time, it is recommended to store it at -80°C after aliquoting to avoid repeated freezing and thawing;
3. If the template is of eukaryotic origin, it is recommended to choose Oligo (dT) 18 Primer, which can be paired with the 3' Poly A tail of eukaryotic mRNA to obtain the highest yield of full-length cDNA;
4. For prokaryotic RNA reverse transcription, please use Random Hexamer Primer or Gene Specific Primer;
5. If reverse transcription is followed by qPCR experiments, Oligo (dT) 18 Primer and Random Hexamer Primer can be used in combination, which can make the cDNA synthesis efficiency of each region of mRNA the same, and help improve the authenticity and repeatability of quantitative results;
6. For your safety and health, please wear a lab coat and disposable gloves for operation.