Peroxidase conjugated anti-Cry j 2 pAb
Peroxidase conjugated anti-Cry j 2 pAb
General information
| Cat. No. | ：FNK-HBL-Ab-2-000P |
| Quantity | ：25 µg, 100 µl/vial (Frozen Liquid) |
| Specificity | ：This antibody was obtained from Cry j 2 antigen (Japanese cedar pollen allergen) immunized Rabbit serum (Japanese White strain). This antibody cross-reacts against Cry j 2 antigen less than 10%. |
| Preparation & form | ：Rabbit IgG was purified from the anti-Cry j 2 rabbit serum by ProteinG Sepharose column chromatography. Then IgG was conjugated with horseradish peroxidase by periodide method. Peroxidase conjugated IgG was prepared in the concentration of 250μg/ml with phosphate- buffered saline containing 1 % bovine serum albumin fractionV |
| Format | ：Protein A / G Affinity Purified |
| Crossing | ：Cryptomeria japonica |
| Immune animals | ：Rabbit |
| Label | ：Unlabeled |
| Class | ：IgG 1 , κ |
| Application | ：This antibody is suitable for enzyme-linked immunosorbent assay. Dilute 1:1000 as a detect antibody. |
| Storage | ：Store below -20°C. Avoid multiple freeze/thaw cycles by storage in appropriate aliquots. |
Description
Pollen, purified antigens (Cry j 1, Cry j 2) and antibodies (anti-Cry j 1 antibody, anti-Cry j 2 antibody) of Japanese Sugi ( Cryptomeria japonica ). It is used in many treatises and boasts a high track record. It is useful for studying Japanese cedar pollen allergy.
It is an antibody produced using Cry j 2 of Japanese Sugi ( Cryptomeria japonica ) as an immunogen.
* This product is for research use. It cannot be used for anything other than research.
What is Japanese cedar pollen?
Two main types of Sugi pollen allergens are known as the causative agents of Sugi pollinosis. One is Cry j 1, a basic protein with a molecular weight of about 40 kDa. Cry j 1 is localized in ubiquitous bodies (particle size of about 0.7 μm) adhering to the surface of Sugi pollen with a particle size of about 30 μm. The other is Cry j 2, a basic protein with a molecular weight of about 37 kDa. Cry j 2 is thought to be localized in the starch granules and endometrium inside Sugi pollen.
References
Wang Seiyaku, et al., "Aerosol Research", 23 , 120-126 (2008).
Hiroshi Yasue, "Ayumi of Medicine", 200 , 358-362 (2002).
References
- Sawatani, M et al. : Immunological and physiological properties of Cry j II, the second major allergen of Japanese cedar pollen. [Article in Japanese] Arerugi, 42(6):738-747, 1993
- Sawatani, M et al.: Enzyme-linked immunosorbent assay for the quantification of Cry j I and Cry j II. [Article in Japanese] Arerugi, 43(3):467-473, 1993
