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Large Plasmid cDNA Library

Product#: LargePlasmidcDNALibrary

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Select [Yeastern Biotech] Yeastern Biotech

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Description
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Description

DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.

While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. The complete genome sequence has been published.

Features

Streptomycin resistant
Prevent DNA methylation
High transformation efficiency

Applications

Suitable for genomic DNA cloning
Allow large inserts
Cloning of methylated cytosine or adenine containing DNA
Blue/white screening

Genotype
F^–endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ^–

Image	Cat.No:	Control Plasmid	Control Efficiency	Storage	Quantity	Link
	YBB-FYE508-10VL	5 μl (10^-4 μg/μl)	>1 x 10⁸ cfu/μg	- 70°C ~ - 80°C	10 Vials
	YBB-FYE508-80VL	5 μl (10^-4 μg/μl)	>1 x 10⁸ cfu/μg	- 70°C ~ - 80°C	80 Vials