Protein Storage Buffer
Protein Storage Buffer
A ready-to-use stabilization buffer with glycerol cryoprotection and TCEP-HCl reduction for short- and medium-term storage of purified proteins. A separate 100x broad-spectrum protease inhibitor cocktail (DMSO-based) is included and is intended to be added freshly to the buffer immediately before use, ensuring maximal inhibitor activity at the time of sample storage while preserving a longer shelf life for the base buffer. Includes a separate 100X broad-spectrum protease inhibitor cocktail.
- Tris base provides a mild, widely compatible environment for many proteins.
- Glycerol helps prevent aggregation and denaturation during cooling, freezing, and thawing.
- TCEP-HCl offers a robust, oxidation-resistant reducing agent, avoiding the odor and instability of DTT at this pH.
- A 100x protease inhibitor cocktail is supplied separately so users can add it to Protein Storage Buffer (or directly to samples) to reach 1x concentration immediately before use.
- Ultrapure Type 1 water (18.2 MΩ·cm)
- Customizable formulation available upon request
- pH7.5 for solution A
- AppearanceClear Solution
- Filtration0.1µm×2 + 0.04µm×2
- Storage
- Shelf Life1 year
Protein Storage Buffer (Base)
- Storage2-8 °C, avoid repeated freeze-thaw cycles
- Shelf Life1 year
- Tris base50 mM
- Sodium Chloride150 mM
- Glycerol10%
- TCEP-HCl2 mM
Protease Inhibitor Cocktail (100X) for Solution A
- Storage-80 °C (long term), avoid repeated freeze-thaw cycles
- Shelf Life1 year
- AEBSF·HCl10 mM
- Aprotinin80 μM
- Bestatin5 mM
- E-64100 μM
- Leupeptin1 mM
- Pepstatin A0.1 mM
Engineered for maximum purity & performance
Standard laboratory reagents filtered at 0.22 µm leave sub-micron particulates, mycoplasma, and aggregates that interfere with sensitive downstream applications. Protein Storage Buffer is manufactured under ISO 13485 quality systems with multi-stage ultra-filtration to eliminate these failure modes.
Glycerol cryoprotection
10% glycerol protects proteins from aggregation and denaturation during cooling, freezing at −20 °C, and thawing.
TCEP-HCl reducing agent
2 mM TCEP-HCl maintains a stable, odorless reducing environment that preserves cysteine residues.
Tris/NaCl physiological base
50 mM Tris and 150 mM NaCl provide a mild, widely compatible environment for stored proteins.
Protease inhibitor cocktail
Separate 100X cocktail (AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A) added fresh for maximum activity.
Sequential filtration system
A sequential filtration process reaching 0.1 µm for clean reagent purity exceeding standard 0.22 µm filtration.
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1
0.1 µm filtration
Removes large particulates, aggregates, and bacteria. Primary guard providing baseline purity exceeding standard 0.22 µm filtration.
Performance vs. conventional reagents
Standard 0.22 µm single-pass filtration leaves particles in the 0.12–0.22 µm range. FluxMPS™ removes these contaminants for cleaner results and higher reproducibility.
Validated applications
- Storage of purified recombinant proteins or protein complexes at 4°C (short term) or -20°C (medium term, in aliquots).
- Storage of concentrated fractions from purification workflows prior to further processing or analysis.
- Sample stabilization for SDS-PAGE, Western blotting, and analytical assays that tolerate Tris, NaCl, glycerol, TCEP, and protease inhibitors.
Quality assurance & logistics
ISO 13485:2016 Certified
Manufactured under certified QMS with full traceability and lot documentation.
Made in USA
All manufacturing, QA testing, and final assembly at DiagnoCine Precision, Totowa, NJ.
Supporting literature
- Wingfield PT. Protein precipitation using ammonium sulfate. Curr Protoc Protein Sci. 2001;Appendix 3:Appendix 3F.
- Carpenter JF, Manning MC, Randolph TW. Long-term storage of proteins. Curr Protoc Protein Sci. 2002;27(1):4.6.1-4.6.6.
