β-Glucosidase HT1, Thermostable
β-Glucosidase HT1, Thermostable
Cat. No. FNK-BGL-54-01
Store at -20°C *Avoid the freeze and thaw.
Size 1 ml
CAS- 9001-22-3
Lot No. 0-H9013
Protein conc. 7.6 mg/ml
Form 20 mM AcONa (pH 6.0), 10 mM NaCl, 1 mM DTT
Activity 109 U/ml
Notes For research use only.
Description
EC No.:3.2.1.74 Substrate: β-1,4-glucan (cellulose) Reaction product : glucose Reaction temperature : ~65℃ Reaction pH : 4.0~6.5
Enzymes work at high temperature with high reactivity The "Thermostable Enzyme".
Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.
Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.
Features of Thermostable Enzyme
- Highly stable and durable for long term use
- High reactivity at high temperature (70 - 100°C)
- Resistance to organic solvent is expected
- Special instruments to inhibit bacterial growth are not required.
- Reaction speficity is much higher compared with chemical reaction
- Speed of reaction can be accelerated
buffer (pH 5.0) and appropriate amount of the enzyme) was incubated at 60 oC and A420 was
measured. One unit is defined as the amount of enzyme producing 1 ?mol of 4-nitrophenol per
1 min from pNP-Glc as a substrate.
