Laccase YK1, Thermostable, 2 ml
Cat. No.: FNK-LAC-27-01-2ml
Stroage -20°C *Avoid repeated freeze and thaw cycles.
Size 2ml
Lot No 1-S301B
Protein conc. 7.0 mg/ml
Form 20 mM Tris-HCl (pH 8.0), 1 mM CuSO4, 1 mM CaCl2
Activity 111 U/ml
Notes For research use only
EC No. 1.10.3.2
Substrate benzenndiol
Reaction product benzosemiquinone
Reaction temperature ~95℃
Reaction pH 3.0~5.0
Description
Enzymes work at high temperature with high reactivity The "Thermostable Enzyme".
Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.
Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.

Features of Thermostable Enzyme
- Highly stable and durable for long term use
- High reactivity at high temperature (70 - 100°C)
- Resistance to organic solvent is expected
- Special instruments to inhibit bacterial growth are not required.
- Reaction speficity is much higher compared with chemical reaction
- Speed of reaction can be accelerated
Activity measurement
Reaction mix (100 mM Sodium acetate buffer (pH 4.0), 1 mM CuSO4, 10 mM 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) and appropriate amount of the enzyme) was incubated at 85 oC and A405 was monitored. One unit is defined as the amount of enzyme oxidizing 1 µmol of ABTS (e408=36.8 mM-1 cm-1 ) per 1 minute using ABTS as a substrate.