FluxMPS™ | Microfluidic-Ready, MMG Protoplast Buffer
FluxMPS™ MMG Protoplast Buffer
A specialized, ready-to-use resuspension buffer for plant protoplasts, providing precise osmotic balance with mannitol, membrane stabilization via MgCl₂, and mildly acidic pH 5.7 buffering with MES. This central working buffer supports high protoplast viability and efficient PEG–Ca²-mediated DNA uptake in transient expression assays such as promoter-reporter studies, signaling readouts, and subcellular localization experiments.
- 4-stage filtration process (0.1 µm → 0.04 µm, followed by sterile 0.1 µm → 0.04 µm)
- Mannitol maintains appropriate osmotic pressure to preserve protoplast integrity during resuspension and transformation steps.
- MgCl₂ enhances membrane stability and supports efficient PEG-mediated DNA uptake.
- MES, adjusted to pH 5.7, matches widely used protoplast transformation protocols, maintaining a stable environment during incubation.
- Ultrapure Type 1 water (18.2 MΩ·cm)
- ISO Class 5 laminar-flow fill & finish
- Customizable formulation available upon request
- pH5.7
- AppearanceClear Solution
- Filtration0.1µm×2 + 0.04µm×2
- Storage2-8 °C
- Shelf Life1 year
Engineered for maximum purity & performance
Standard laboratory reagents filtered at 0.22 µm leave sub-micron particulates, mycoplasma, and aggregates that interfere with sensitive downstream applications. FluxMPS™ MMG Protoplast Buffer is manufactured under ISO 13485 quality systems with multi-stage ultra-filtration to eliminate these failure modes.
Mannitol osmotic support
0.4 M mannitol stabilizes wall-less protoplasts, minimizing osmotic swelling and lysis during resuspension and transformation.
MgCl₂ membrane support
15 mM MgCl₂ supports membrane organization and coordinates with PEG-Ca² solutions for efficient DNA binding and uptake.
MES pH 5.7 environment
4 mM MES at pH 5.7 matches the empirically optimized conditions for high protoplast viability and transformation efficiency.
Transformation-ready
Designed for use immediately upstream of PEG-Ca² DNA delivery in promoter-reporter, subcellular localization, and functional screening assays.
Quadruple-stage filtration system
A serial four-stage sequential filtration process reaching 0.04 µm—the only commercially available buffer at this level of particle removal.
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1
0.1 µm Pre-filtration I
Removes large particulates and aggregates. Primary guard to extend downstream filter lifetime.
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2
0.04 µm Pre-filtration II
Removes fine particulates, bacteria, and mycoplasma (0.1–0.3 µm)—a key step absent in standard 0.22 µm filtration.
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3
0.1 µm Sterile-filtration I
Second-pass removal of residual contaminants and bioburden with full redundancy.
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4
0.04 µm Sterile-filtration II — Final Polish
Ultimate filtration for particle-free, mycoplasma-free product. Final fill in validated ISO Class 5 laminar-flow workstation.
Performance vs. conventional reagents
Standard 0.22 µm single-pass filtration leaves particles in the 0.05–0.22 µm range. FluxMPS™ removes these contaminants for cleaner results and higher reproducibility.
Validated applications
- Resuspension of plant protoplasts prior to PEG-Ca²?-mediated DNA transformation.
- Adjusting protoplast concentration for electroporation or other transfection procedures (after protocol-specific optimization).
- Short-term holding of protoplasts during counting, viability checks, or setup of transformation reactions.
Protocol & usage instructions
- After washing protoplasts (often in W5 buffer), gently pellet and resuspend in MMG Protoplast Buffer to the desired cell density (e.g., ~1-3 × 10? protoplasts/mL, per protocol).
- Use MMG as the standard medium to mix protoplasts with plasmid DNA before addition of PEG-Ca²? solution.
- Keep protoplasts on ice or at room temperature according to your specific protocol; many workflows keep MMG steps at room temperature for optimal transformation efficiency.
- Limit protoplast residence time in MMG to the period required for transformation setup and immediate downstream steps.
Composition & specifications
| Component |
|---|
| Mannitol |
| Magnesium Chloride |
| MES |
Quality assurance & logistics
ISO 13485:2016 Certified
Manufactured under certified QMS with full traceability and lot documentation.
Made in USA
All manufacturing, QA testing, and final assembly at DiagnoCine Precision, Totowa, NJ.
Supporting literature
- Yoo SD, Cho YH, Sheen J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc. 2007;2(7):1565-1572.
- Wu FH, Shen SC, Lee LY, et al. Tape-Arabidopsis Sandwich: a simpler Arabidopsis protoplast isolation method. Plant Methods. 2009;5:16.
- Sheen J. Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol. 2001;127(4):1466-1475.
