Citrate Buffer [0.1X]
Cat. No. DCP-CB0.1X
Size: 500 mL
Buffer Solution 0.1M
pH: 3.0
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
- World’s cleanest buffer for all cell and molecular biology experiments
Description:
Citrate buffer is a versatile and widely used buffer solution in biochemistry and molecular biology. It is effective in immunohistochemistry (IHC) for antigen retrieval from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The buffer works by breaking protein cross-links formed during tissue fixation, thereby unmasking antigens and epitopes and enhancing the staining intensity of antibodies. It typically provides excellent staining with a very low background, resulting in a better signal-to-noise ratio. Citrate acts as a chelating agent, particularly for calcium ions. This property can benefit some applications but may interfere with reactions requiring specific metal ion concentrations. It maintains a relatively stable pH over a range of temperatures, making it suitable for applications involving temperature changes.
Advantages:
- It can enhance stain intensity without increasing the background signal.
- It tends to cause less tissue damage or morphological changes.
- It works well with many different antibodies and can detect a wide range of antigens in various tissues.
Application:
- Citrate buffer helps prevent base hydrolysis during RNA extraction.
- It is commonly used in immunohistochemistry for heat-induced epitope retrieval (HIER) to reverse the loss of antigenicity in formalin-fixed paraffin-embedded tissues.
- DNA fluorescent in situ hybridization.
- Enzymatic reactions and chromatography
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 3.0
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 18 months
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
