SK-Mill : Freezing and Crushing Equipment

Super strong crushing with bullets

Suppresses decomposition and denaturation of target substances at low temperatures

Since it is a closed type, it is safe for viable bacteria and toxicity. Its small size makes it easy to freeze and crush.

Those with small hands are also recommended. It can be easily crushed just by shaking it by hand. Depending on the sample, shake it by hand and crush it in about 30 seconds, extract RNA, DNA, and protein. Freeze crushing by shaking it up and down by hand, and crushing is completed in just about 30 seconds.

Compared to beads, it has a stronger impact and can be crushed strongly, and since the sample is handled at an extremely low temperature, it can be crushed by suppressing the decomposition and denaturation of the target substance, unlike crushing when using a homogenizer or ultrasonic waves.

Since the sample is handled in a sealed state, even a sample that is dangerous to the human body can be handled with confidence.


 

Features of SK-Mill, a freezing and crushing device

Freezing and crushing equipment SK mill specifications and types

 

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Description

 

This apparatus has been developed for the purpose of crushing various frozen tissues easily. The conventional methods is to crush tissues in mortar using liquid nitrogen, which are really troublesome. In contrast, Our SK-Mill can now freeze-crush them easily. You can now prevent your samples from scattering and also from contamination because our SK-Mill is designed to use disposable closed type plastic Sample Tubes. Improved efficiencies will speed up your entire process!
 

SUCCEESSFULLY APPLIED FOR (as of :2003.9.30)

• Plant leaves (tobacco plant,rice plant and adzuki bean) STRUCTURE
• Yeasts
• Arabidopsis thaliana
• Rice stalks and roots
• Mouse liver, cortex and hippocampus
• Mushroom
• Rat skeletal muscle
• Nematode
• Fungi (hyphae and spores)
• Filamentous fungi
• Placenta 

 

 Example1: freezing-crushing of yeast

 
1.Culture yeast (S. cerevisiae) in YPD medium until exponential growth phase.
2.Collect fungi from 1ml culture in 2ml sample tube using centrifuge.
3.Then, freeze and crush them by SK MILL.
4.Eliminate fungal residue and recover nucleic acid by phenol extraction and ethanol precipitation.
5.Apply it to electrophoresis.

Example2 : RNA extraction from green leaves of rice

 
1.Cut green leaves of rice ( ca. 1 gram) into small pieces by scissors.
2.Then, freeze and crush them by SK MILL.
3.Extract RNA by CTAB ( cetyltrimethylammonium bromide) method.
4.Apply it to electrophoresis. 
 

Specification of SK-Mill (see the above table for more info.)