CAPS Transfer Buffer
Cat No.: DCP-CAPSTB0.5X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
READY-to-USE 0.5X CAPS (3-(Cyclohexylamino)-1-propanesulfonic acid) buffer is a transfer buffer used in Western blotting, particularly for certain specialized applications. It has a high pH of 11 which makes it effective for transferring high molecular weight proteins and basic proteins with high isoelectric points. The high pH helps to maintain protein solubility during transfer, especially for larger proteins that may be prone to precipitation. Additionally, the high pH helps to maintain these proteins in a negatively charged state, promoting their migration towards the positively charged electrode during transfer. It helps to dissociate SDS from proteins more effectively than buffers with lower pH. SDS can hinder protein from binding to membranes, particularly for smaller proteins due to SDS-coated proteins not interacting effectively with the hydrophobic surfaces of the membranes and reducing transfer efficiency. Methanol helps to minimize the gel swelling during transfer to ensure the dimensional stability of the gel and preserves protein resolution. It also helps to ensure that the proteins remain soluble during the transfer process and prevent precipitation.
Applications
- Western Blotting of High Molecular Weight Proteins: CAPS buffer is particularly suitable for transferring proteins greater than 20 kDa.
- Protein Sequencing: subsequent protein sequencing, as it doesn't interfere with automated protein sequencers using the Edman principle.Presequencing often used for transfers prior to N-terminal sequencing.
- Transfer of Basic Proteins: recommended for blotting of basic proteins.
Advantages:
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CAPS buffer works well with both nitrocellulose and PVDF membranes, allowing for efficient protein adsorption.
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Presence of methanol helps to increase the hydrophobicity of the membrane, enhancing protein adsorption
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Efficient Transfer:When used in a discontinuous system with Tris, it can facilitate efficient transfer of proteins from gel to membrane.
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Adjustable pH: The pH can be adjusted to 10.5-11.0, which is optimal for many protein transfer applications.
Composition:
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 11.0
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
