Our Long Range PCR Service specializes in the amplification of large DNA fragments, typically over 5 kb and up to 30 kb, using advanced high-fidelity polymerases and optimized protocols. This service is ideal for researchers requiring the analysis, cloning, or sequencing of extended genomic regions, detection of structural variations, or amplification of GC-rich and difficult templates. We employ a combination of high-performance enzymes and proprietary reaction buffers to achieve reliable amplification of long targets with high accuracy and minimal error rates. Applications include genome mapping, structural variant analysis, gene cloning, detection of trinucleotide repeat expansions, and more.
Important: For the safety of our staff and compliance with regulations, we accept only non-pathogenic samples. Please do not submit any materials classified as pathogenic, hazardous, or requiring special biosafety containment.
How Our Service Works
1. Consultation: Submit your project details and sample information using the request form below. Our team will review your requirements and clarify any details as needed.
2. Optimization: We design and validate primer sets and optimize PCR conditions for your target region and template.
3. Amplification: Using high-fidelity, long-range PCR enzymes, we amplify your specified DNA fragment.
4. Quality Control: Amplified products are assessed by gel electrophoresis and, if requested, sequence-verified.
5. Delivery: Receive your purified PCR products and data in your preferred format, shipped securely to your address.
If we are unable to obtain any results from your sample, we offer a 50% refund of your service fee.
Please consult with scientific team first prior to sending in samples.
Frequently Asked Questions
Q: What fragment sizes can you amplify?
A: We routinely amplify DNA fragments from 5 kb up to 30 kb or more, depending on template quality and complexity.
Q: What templates do you accept?
A: We work with high-quality, non-pathogenic DNA templates, including plasmid DNA, bacterial DNA, cDNA, and certain genomic DNA samples.
Q: What are the quality requirements for DNA samples?
A: High molecular weight, purified DNA is required for optimal results. Degraded or impure samples may result in poor amplification.
Q: Can you work with GC-rich or difficult templates?
A: Yes, we can use specialized buffers and polymerases designed for GC-rich and challenging templates.
Q: How long does the process take?
A: Turnaround time is typically 1-2 weeks, depending on project complexity and verificiation requirements.
Q: Do you provide sequence verification?
A: Sequence verification is available upon request.
Q: Do you accept pathogenic samples?
A: No. For safety and compliance, we accept only non-pathogenic samples.
Please note that prepayment is required for all PCR services.
We strive to deliver accurate and timely results. However, in the rare event that no result is obtained from your sample, we will refund 50% of the service fee as a courtesy.
Additional Information
Non-Pathogenic Samples Only: For the safety of our staff and compliance with laboratory regulations, we do not accept pathogenic or clinically significant microorganisms. Confidentiality: All client data and results are handled with strict confidentiality and secure web portal access is available for result retrieval. Expert Support: Our team of experienced microbiologists is available to provide guidance on sample submission, interpretation of results, and follow-up investigations. Industries Served: We support pharmaceutical, food and beverage, cosmetics, environmental, and industrial sectors.
Helpful Resources Available:
For your convenience, documents such as our Sample Submission Form and detailed Shipping & Handling Guidelines can be found under the FAQ and Manual tabs. Please refer to these sections for important information and downloadable forms to assist with your service requests.
Request a Quote
Tell us about your project! Fill out the form below, or email us at support@diagnocine.com. Sign in to your Google Account to access the content below.
Fields marked with * are mandatory.
After submitting, a representative will contact you to discuss your project in detail and provide a formal quotation.
