Bicarbonate / Carbonate Coating Buffer [1X]
Cat No.: DCP-BCB100X
Size: 1000 mL & 500 mL
Non-Sterile; Ultrapure, Filtered 0.1-micron Once and 0.04 Micron Membrane Filtered Once
Description
The bicarbonate/carbonate coating buffer is commonly used for coating proteins and antibodies onto plastic surfaces, such as microtiter plates and tubes, for various immunoassay techniques like ELISA, EIA, and RIA. Some examples of applications are coating microtiter plates, plastic tubes, flasks or dishes for various protein and antibody conjugation procedures. Sometimes, it is used as a small intestine buffer in the medical research field. It is a coupling buffer when immobilizing proteins like agarose on chromatography media. It has an alkaline pH, creating a negatively charged environment on the surface of polystyrene plates/tubes. Proteins and antibodies are positively charged at high pH and attracted to the negatively charged surface.
Advantages:
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It helps in the adsorptive immobilization of proteins and antibodies onto the plastic surface.
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It helps to maintain a stable pH environment.
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It enables various immunoassays and protein-binding applications.
Composition:
| Ingredients |
Concentration |
| Na2CO3 |
0.74 M |
| NaHCO3 |
0.26 M |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 9.5
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Sterility: Filtered 0.1 micron Once + 0.04 micron in a non-sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
