Anti-p53(pSer46), Phosphorylated, Mouse-Mono(36)

p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Among the phosphorylation sites, Ser46 is phosphorylated when DNA damage is so severe as to become unrepairable, which leads to apoptosis by activating transcription of proapoptotic genes such as p53AIPI As to the kinase of phosphorylation of Ser46, involvement of DYRK2 and ATM have been implicated

 

	Fig.1. Identification of Ser46-phosphorylated p53 protein by western blotting. Samples: Crude cell extracts of MOLT-4 untreated (left lanes) and treated with adriamycin for 24 h (right lanes). The left panel is the result with our product and the right panel is the one obtained with the product of our competitor. The lower panel is the whole p53 protein identified by omnipotent anti-p53 antibody
	Fig.2. Kinetics of phosphorylation of p53 at Ser46 after X-ray irradiation . Samples of U2OS cells (human osteosarcoma) were taken at the indicated times after X-ray irradiation at 10 Gy and analyzed by western blotting with anti-p53 p-S46 antibody (#36) and anti-p53 antibody (non BioAcademia). Primary antibodies were diluted with “ Can Get Signal “ signal enhancer (Toyobo, Osaka).
	Fig.3. Kinetics of phosphorylation of p53 at Ser46 after UV-irradiation. Samples of MCF7 cells (human beast cancer cell line) were taken at the indicated times after UV-irradiation at 20 J/m2 and analyzed by western blotting with anti-p53 p-S46 antibody (#36) and anti-p53 antibody (non BioAcademia).Primary antibodies were diluted with “ Can Get Signal “ signal enhancer
	Fig.4. Immunohistochemistry of stomach cancer.

This product was used in the following publications.