Anti-Rb phospho-Ser795 antibody, monoclonal (28B5)
Anti-Rb phospho-Ser795 antibody, monoclonal (28B5)
General Information
| Cat. No. | ：FNK-71-171 |
| Size | ：50 ug |
| Antigen | ：Human |
| Host Species | ：Mouse |
| Cross Reactivity | ：Human |
| Label | ：Unlabeled |
| Clone | ：28B5 |
| Immunogen | ：A synthetic peptide containing phospho-Ser795 of human Rb |
| Specificity | ：Specific to human Rb phosphorylated at Ser795. Not tested for other species. Can detect endogenous levels of Rb phosphorylated at Ser795 in most cell lines. |
| Form | ：Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized |
| Isotype | ：Mouse IgG2a (κ) |
| Application | ：
|
| Storage | ：-20℃ (long period, -70℃) |
| Data Link | ：UniProtKB/Swiss-Prot P06400 (RB_HUMAN) |
Description
Retinoblastoma protein (Rb), the tumor suppressor product of the retinoblastoma susceptibility gene, is a 110 kDa protein that functions as a negative regulator of the cell cycle by arresting cells in the G1 phase and halting inappropriate cell proliferation (1). At the transcriptional level, Rb protein exerts its growth suppressive function by binding to transcription factors including E2F1, PU1, ATF2, UBF, Elf-1 and c-Abl (2-4). Loss of Rb function leads to uncontrolled cell growth and tumor development and is found in all retinoblastomas and in a variety of other human malignancies. The ability of Rb protein to inhinbit transcription and cell cycle progression is inactivated by phosphorylation, which is catalyzed by the cyclin-dependent protein kinases.
Fig.1 Specificity of the monoclonal antibody (28B5) to the phosphorylated Rb at Ser795 as demonstrated by Western blotting.
Crude extracts of human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged wild-type Rb (lane 1), Rb (S795A) (lane 2) or Rb (T821A) were immuno-precipitated with anti-Myc antibody and the precipitates were analyzed by Western blotting with monoclonal antibody 28B5.(Data provided by Dr. Y. Inoue at the JFCR Cancer Research Institute, Tokyo)
References
- Sherr CJ “Cancer cell cycles”Science 274: 1672-1677 (1996) PMID:8939849
- Nevins JR “E2F: a link between the Rb tumor suppressor protein and viral oncoproteins” Science 258: 424-429 (1992) PMID: 1411535
- Welch PJ & Wang JY “A C-terminal protein-binding domain in the retinoblastoma protein regulates nuclear c-AbI tyrosine kinase in the cell cycle”Cell 75: 779-790 (1993) PMID: 8242749
- Hu QJ et al ”The regions of the retinoblastoma protein needed for binding to adenovirus E1A or SV40 large T antigen are common sites for mutations”EMBO J 9:1147-1155 (1990) PMID: 2138977
Aliases for RB1 Gene
- RB Transcriptional Corepressor 1 2 3 5
- Protein Phosphatase 1, Regulatory Subunit 130 2 3
- Prepro-Retinoblastoma-Associated Protein 2 3
- Retinoblastoma-Associated Protein 3 4
- Retinoblastoma 1 2 3
- PPP1R130 2 3
- P110-RB1 3 4
- P105-Rb 3 4
- Pp110 3 4
- PRb 3 4
- RB 2 3
- Exon 17 Tumor GOS561 Substitution Mutation Causes Premature Stop 3
- GOS563 Exon 17 Substitution Mutation Causes Premature Stop 3
- Retinoblastoma Suspectibility Protein 3
- Osteosarcoma 2
- OSRC 3
- RB1 5
- Rb 4
