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Anti-Nup153 antibody, rat monoclonal (R4C8)

Product#: FNK-70-315

$346.79

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Anti-Nup153 antibody, rat monoclonal (R4C8)

General information

Cat. No.	：FNK-70-315
Size	：200 µg
Antigen Species	：Rat
Host Species	：Rat
Immunogen	：Recombinant GST-fused rat Nup153 (610-1191aa)
Isotype	：Rat IgG2a kappa
Epitope	：610-1191 aa (Zn finger and FG repeats domain)
Form	：Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized
Reactivity	：Reacts with human, mouse, rat, hamster and monkey
Application	：Western blotting (1/200) ：Immunofluorescent staining (1/50) ：ELISA Other applications have not been tested. ：Additional comments: When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well (Fig. 2 & 3).
Storage	：Shipped at 4℃ or -20℃, and upon arrival, aliquot and store at -20℃.
Data Link	：UniProtKB/Swiss-Prot P49790 (NU153_HUMAN), UniProtKBP49791(NU153_RAT)

Description

The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.


Fig.1 Detection of Nup153 by Western blotting with antibody R4C8. Sample is the nuclear membrane fraction of HeLa cells (Left) and NIH3T3 cells (Right).	Fig.2 Immunofluorescent staining of HeLa cells with antibody R4C8.

	Fig.3 Immunofluorescent staining of rat neuron with antibody R4C8. The dots are Nup153.

References (This antibody has been used in the following publications.)

Iino H et al. Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells. Genes to CellsVolume 15, Issue 6. IF (hamster)
Maeshima K et al. Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase. Nature Structural & Molecular Biology volume 17, pages1065–1071 (2010).IF, WB (human)

Aliases for NUP153 Gene