Anti-HIV-1, Gag p17, Guinea Pig-Poly

HIV-1 Gag p17 is the matrix protein of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction (1). 

The product is prepared by immunizing rabbit with recombinant p17 bprotein which was over-expressed in E. coli with a plasmid carrying the Gag p17 coding region of HIV-1 virus, subtype B (2), and was highly purified by several steps of chromatography (3, 4).

Using this antiserum in Western blotting, the bands of 17 kD and 55 kD corresponding respectively to HIV-p17 and its precursors p55 were observed in the extract of the AIDS virus infected cells (Fig. 1).
 

1) It can be used in Western blotting or ELISA for the purpose of HIV-1 p17 detection and trtration.

 

Fig.1 Detection of HIV-1 p17 and its p55 precursor proteins by Western blotting using the anti p17 antibody  Lane 1: Extract of MT4 cells Lane2: Extract of MT4 cells infected with HIV-1(LAI strain).  The antiserum was diluted 2,500 fold before use.