Anti- KDM5A / RBP2 / JARID1A antibody, mouse monoclonal (9A6)
Product#: FNK-71-175
$346.79
Availability:
Ships in 1-2 Weeks
Anti- KDM5A / RBP2 / JARID1A antibody, mouse monoclonal (9A6)
General Information
| Cat. No. | ：FNK-71-175 |
| Size | ：50 ug |
| Antigen | ：Human |
| Host Species | ：Mouse |
| Label | ：Unlabeled |
| Clone | ：9A6 |
| Immunogen | ：A synthetic peptide corresponding to human RBP2, amino acids 1416-1434. |
| Reactivity | ：Human and mouse RBP2. Can detect endogenous levels of RBP2. |
| Form | ：1mg/ml in PBS, 50% glycerol, filter-sterilized |
| Isotype | ：Mouse IgG2a kappa |
| Purification | ：IgG fraction is purified from supernatant of the hybridoma cell culture in serum-free medium under mild conditions by proprietary chromatography. |
| Application | ：
|
| Storage | ：Ship at 4℃ or -20℃ and stored at -20℃ |
| Data Link | ：UniProtKB/Swiss-Prot P29375 (KDM5A_HUMAN) ：UniProtKB/Swiss-Prot Q3UXZ9 (KDM5A_MOUSE) |
Description
RBP2 was originally identified as a retinoblastoma binding protein. It is also known as JARID1A (Jumonji, AT rich interactive domain 1A). RBP2 plays both negative and positive roles in RB-mediated transcriptional activation, depending on the kinds of genes and regulates differentiation by its function as an H3K4 histone demethylase (1, 2 & 3).
Figure Identification of RBP2 in crude cell extracts by Western blotting with antibody 9A6.
Samples:
1. HeLa control siRNA
2. HeLa RBP2 siRNA
3. MCF7
4. U2OS
5. NIH3T3 6. J1 (mouse ES)
A. SDS-PAGE with CBB staining B. Western blotting (detection: ECL)
References
- Lopez-Bigas N et al “Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals atranscriptional program controlling differentiation” Moll Cell 31: 520-530 (2008) PMID: 18722178
- Klose RJ et al “The retinoblastoma binding protein BRP2 is an H3K4 demethylase” Cell 128: 889-900 (2007) PMID: 17320163
- Christensen J et al “RBP2 belongs to a family of demethylases, specific for tri- and dimethylated lysine 4 on histone 3” Cell 128:1063-1076 (2007) PMID: 17320161
Aliases for RBP2 Gene
- Retinol Binding Protein 2 2 3 5
- CRBP2 2 3 4
- Cellular Retinol-Binding Protein II 3 4
- Retinol-Binding Protein 2 3 4
- CRABP-II 2 3
- CRBP-II 3 4
- CRBPII 2 3
- RBPC2 2 3
- Retinol-Binding Protein 2, Cellular 2
- Retinol Binding Protein 2, Cellular 3
- RBP2 5
