Anti Human GCN mouse monoclonal antibody (H7921)
Product#: FNK-PP-H7921-00
$501.52
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Anti Human GCN mouse monoclonal antibody (H7921)
GCNF: Germ cell nuclear factor
General information
| Cat. No. | ：FNK-PP-H7921-00 |
| Size | ：100 ul |
| Antigen Species | ：Human |
| Host Species | ：Mouse |
| Cross Reactivity | ：Human |
| Purification | ：Ammonium sulfate fractionation. |
| Clone No | ：H7921 |
| Lot. | ：A-1 |
| Concentration | ：1 mg/mL |
| Ig Class | ：G2a |
| Nomenclature | ：NR6A1 |
| Application | ：Western Blot (1 ug/mL), Non reducing Western Blot (3 ug/mL) |
| Specificity |
：This antibody specifically recognizes human GCNF. Not yet tested in other species.
* In WB applications, a 35kDa non-specific band has been observed. |
| Storage | ：Store at 2 - 8 ºC up to one month. For long-term storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in a frost-free freezer is not recommended. |
| Shipping Condition | ：Dry Ice |
| Form | ：Physiological saline with 0.1% NaN3 as a preservative |
| Genbank | ：U64876 |
Description
GCNF(NR6A1) termed germ cell nuclear factor is an orphan receptor which has no paralogous observed in mammal. The expression is observed only in germ cells of the testis, the spermatids and late-stage spermatocytes. GCNF may have a role in regulating meiotic and post-meiotic stage of germ cells.
Human Diseases
- Infertility: GCNF plays a critical role in spermatogenesis, and its dysregulation can lead to male infertility due to impaired germ cell development.
- Cancer: Altered expression of GCNF has been linked to various cancers, suggesting its involvement in tumorigenesis and cancer progression.
- Developmental Disorders: As a transcriptional regulator, GCNF is essential for normal embryonic development, and mutations may contribute to congenital anomalies.
Cellular Signaling Pathways
- Meiosis Regulation: GCNF is involved in the transcriptional regulation during meiosis, influencing gene expression necessary for germ cell development.
- Retinoic Acid Signaling: GCNF can repress retinoic acid signaling pathways, which are crucial for proper embryonic development and differentiation.
- Nuclear Receptor Signaling: As an orphan nuclear receptor, GCNF interacts with other nuclear receptors to modulate gene expression related to cell fate and differentiation.
Origin
Produced in BALB/c mouse ascites after inoculation with hybridoma of mouse myeloma cells (NS-1) and spleen cells derived from a BALB/c mouse immunized with Baculovirus-expressed recombinant human GCNF(28-58 aa) .
Note
Sodium azide may react with lead and copper plumbing to form explosive metal azides. Flush with large amounts of water during disposal.
Aliases for NR6A1 Gene
- Nuclear Receptor Subfamily 6 Group A Member 1 2 3 4 5
- RTR 2 3 4
- Retinoid Receptor-Related Testis-Specific Receptor 3 4
- CT150 2 3
- GCNF1 2 3
- HGCNF 3 4
- GCNF 3 4
- HRTR 3 4
- Retinoic Acid Receptor-Related Testis-Associated Receptor 3
- Nuclear Receptor Subfamily 6, Group A, Member 1 2
- Germ Cell Nuclear Factor Variant 1 3
- Germ Cell Nuclear Factor 4
- NR6A1 5
- NR61 3
References
- Yang, Genyan, Yong-Lian Zhang, et al. “Analysis of Germ Cell Nuclear Factor Transcripts and Protein Expression during Spermatogenesis1.” OUP Academic, Oxford University Press, 1 May 2003, academic.oup.com/biolreprod/article-abstract/68/5/1620/2683591?login=false&redirectedFrom=fulltext.
- School Feeding Resource Library.” GCNF, 8 Jan. 2024, gcnf.org/resources/.
- Global Child Nutrition Foundation.” GCNF, 13 Sept. 2024, gcnf.org/.
