HiDi® DNA polymerase, 1000 U

Product#: MBC-#9001M
$368.00
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HiDi® DNA polymerase, 1000 U


Cat. No.:  MBC-#9001M


size:  1000 U



 
Description

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant suitable for SYBR chemistry, specially optimized for assays in which high discrimination is required. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.


Further Specification
Content

S pack: 250 U, 5 U/µl, 1 x 50 µl HiDi® DNA polymerase; 1 x 1.25 ml 10x HiDi reaction buffer

M pack: 250 U, 5 U/µl, 1 x 200 µl HiDi® DNA polymerase; 2 x 1.25 ml 10x HiDi reaction buffer


Storage and Shipping
Storage -20°C
Shipping Cold packs
 
References

Drum, M., Kranaster, R., Ewald, C., Blasczyk, R., & Marx, A. (2014). Variants of a Thermus aquaticus DNA polymerase with increased selectivity for applications in allele-and methylation-specific amplification. PloS one, 9(5), e96640.

Barraud, N., Létoffé, S., Beloin, C., Vinh, J., Chiappetta, G., & Ghigo, J. M. (2021). Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress. npj Biofilms and Microbiomes, 7(1), 1-11.

Miotto, O., Sekihara, M., Tachibana, S. I., Yamauchi, M., Pearson, R. D., Amato, R., ... & Mita, T. (2020). Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea. PLoS pathogens, 16(12), e1009133.

Yang, Z., Le, J. T., Hutter, D., Bradley, K. M., Overton, B. R., McLendon, C., & Benner, S. A. (2020). Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems. Biology Methods and Protocols, 5(1), bpaa004.

Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., ... & Mashimo, T. (2019). CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nature communications, 10(1), 1-13.

Madhuri, S., Bártulos, C. R., Serif, M., Lepetit, B., & Kroth, P. G. (2019). A strategy to complement PtAUREO1a in TALEN knockout strains of Phaeodactylum tricornutum. Algal Research, 39, 101469.





 

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