Accuris AccuFi High Fidelity Master Mix, 2x conc., 500 reactions
Product#: BM-PR1002-HFHS-500
$640.00
Availability:
Ships in 1-2 Weeks
Accuris AccuFi High Fidelity Master Mix, 2x conc., 500 reactions
Cat.No: BM-PR1002-HFHS-500Storage: Immediately store at -20°C. Avoid excessive freeze/thaw cycles. When stored as directed, this product will retain its activity for 12 months from date of receipt.
Size: 500 x 25µl rxns
Limiation of Use
For research purposes only. Not intended for therapeutic or diagnostic use.Description
Accuris AccuFi HFHS PCR Mix is optimized 2X mastermix that includes AccuFi High Fidelity polymerase, a proprietary reaction buffer system, MgCl2 , and ultra-pure dNTPs. The engineered polyerase exhibits rapid extension kinetics (~10 s/kb), enabling efficient fast cycling protocools while maintaining high processivity. The formulation supports robust long-range amplification, with reliable performance on templates exceeding 15 kb.The enzyme possesses both 5’→3’ DNA polymerase activity and intrinsic 3’→5’ exonuclease proofreading activity, resulting in ultra-low error rate (below the detection limit) compared to Taq DNA polymerase. The high fidelity of the enzyme supports applications where sequence integrity is critical, such as molecular cloning, next-generation sequencing (NGS) library construction, and site-directed mutagenesis.
The optimized buffer system enhances amplification efficiency across challenging templates, including GC-rich and partially impure DNA samples, while maintaining high yield and and specificity with minimal reaction optimization.
Highlights
- Ultra-high fidelity: Exhibits highly accurate DNA synthesis supported by coupled 5’→3’ DNA polymerase activity and intrinsic 3’→5’ exonuclease proofreading.
- Hot-DNA polymerase: minimizes nonspecific amplification, reduced background and enhances reproducibility in PCR
- Strong amplification performance: Supports efficient amplification across challenging templates, including GC-rich sequences, low-quality, and long-range targets, while maintaining high yield.
- Fast extension rate: Delivers rapid DNA synthesis at approximately 1 kb/10 seconds, supporting accelerated PCR cycling.
PCR Master Mix Guidelines:
| Component | 25 uL reaction | 50 uL reaction | Final concentration* |
| AccuFiTM PCR Mix | 12.5 μL | 25 μL | 1X |
| Forward Primer (10 μM) | 1.0 μL | 2.0 μL | 400 nM |
| Reverse Primer (10 μM) | 1.0 μL | 2.0 μL | 400 nM |
| DNA Template | < 100 ng cDNA
<500 ng genomic DNA
|
variable | |
| PCR-grade H2O | Up to 25 μL | Up to 50 μL | |
PCR Cycling Guidelines:
| Stage | Temperature | Time | # Cycles |
| Initial Denaturation | 95°C | 1 minute | 1 |
| Denaturation | 95°C | 15 seconds | |
| Annealing* (varies) | 60-65°C | 15 seconds | 25-40 |
| Extension* | 72°C | 30 seconds per kb |
**General Guidelines is located under Manual
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