iMatrix 221 (6 X 175ug)

iMatrix 221 (6 X 175ug)

Cat. No.:		FNK-892062
Size:		6 X 175ug
Description:		iMatrix-221 is a recombinant human laminin-221 E8 fragment protein expressed in Chinese Hamster Ovary (CHO)-S cells. iMatrix-221 contains the integrin-binding site of the laminin-221 molecule. iMatrix-221 is a useful cell culture substrate for proliferation and differentiation of cardiomyocytes and skeletal muscule cells. iMatrix-221 is also useful for the culture of other cells adhering to laminin-221.
Content:		Recombinant human laminin-221 E8 fragment protein in PBS(-)
Concentration:		0.5 mg/mL
Amount:		175 μg / 0.35 mL / tube  (1050 μg / 6 tubes)
Storage:		Store at 2°C to 15°C, protect from light.
Expiration date:		The shelf life is 2 years from the date of manufacture. The expiration date is printed on the outer carton.
Activity:		The dissociation constant (Kd) for the binding with integrin α7X2β1 is 10 nM or less.
Methods of use:		By the following method, iMatrix-221 can be coated onto a culture vessel. The optimum coating density may differ by cell-type, cell-line, medium selected, or purpose. Insufficient coating density may result in the detachment of cells and varied cell conditions while the excessive coating density may lead to difficulty in detaching cells for passage. Determine the optimal coating density. 0.5 μg/cm2 is a standard but test between 0.1 and 1.5 μg/cm2. Dilute iMatrix-221 with PBS(-). Use the diluted iMatrix-221 immediately. To coat with 0.5 μg/cm2 onto a 6-well plate with 9.6 cm2/well, dilute 9.6 μL of iMatrix-221 with 2 mL of PBS(-) per well. Place the diluted iMatrix-221 into a culture vessel and incubate either at 37°C for 1 h, or at room temperature for 3 h, or at 4°C overnight. Aspirate the coating solution. Then, immediately seed your cells. Do not allow the coated surface to dry. *If you face difficulties in detaching cells for passage, re-adjust the conditions (e.g., reduce the coating density).
References:		Nishiuchi R. et al. (2006), Matrix Biol. 25 (3): 189-97 Taniguchi Y. et al. (2009), J. Biol. Chem. 284 (12): 7820- 31 Israeli-Rosenberg S. et al. (2014), Circ. Res. 114 (3): 572-86 Ishii K. et al. (2018), Stem Cell Reports 10 (2): 562-82
Caution:		For research use only. Not intended for human use. In the event of accidental ingestion or contact with the eyes, immediately wash the affected area and seek medical attention.

The Biology behind the Technology

Laminins are an essential component of the extracellular matrix and make up a large portion of the basement membrane. They support and regulate cell growth, motility and differentiation.  Laminins are trimeric proteins made up of a, /3 and y chains and the nomenclature of the different isoforms is based on the type of chain that it contains. 

Of note, lamimin 511 is expressed during embryogenesis and research by Professor Kiyotoshi Sekiguchi at Osaka University in Japan has unveiled that the cellular adhesion site is located in what is named the E8 region. Through his work, it was also found that the laminin E8 fragment provided superior performance as a substrate compared to full-length laminin. 

Laminins are cell adhesion proteins

Combination of laminins and cell type in vivo

A series of recombinant human laminins as various cell culture substrate.

Laminin is one of the extra cellular matrixes (ECM) located in the basement membrane of animals, and play an important role for cell adhesion and proliferation. Laminin is composed of three parts ; alpha, beta, and gamma chain, and 15 types of laminin has been already known.

iMatrix 221: For cardiomyocytes or skeletal muscule cells culture

iMatrix 221 is a useful cell culture substrate for proliferation and differentiation of cardiomyocytes and skeletal muscule cells. This is a recombinant human laminin 221 E8 fragment protein which contains the integrin binding site of the laminin 221 molecule.

iMatrix™ Cell Culture Substrates

Product	Name	Product ID	Description	Usage	Size	Link
	iMatrix-221	FNK-892061	Recombinant Human Laminin-211 E8 Fragments	Cardiomyocytes	2 × 175 μg
		FNK-892062			6 × 175 μg
	iMatrix-411	FNK-892041	Recombinant Human Laminin-411 E8 Fragments	Endothelial cells	2 × 175 μg
		FNK-892042			6 × 175 μg
	iMatrix-511	FNK-892011	Recombinant Human Laminin-511 E8 Fragments	Pluripotent Stem Cells	2 × 175 μg
		FNK-892012			6 × 175 μg
	iMatrix-511 Silk	FNK-892021	Recombinant Human Laminin-511 E8 Fragments, expressed in Silkworm	Pluripotent Stem Cells	6 × 175 μg