What is the difference between Ultrapure Water, Type 1 and the DEPC-treated grade?

Both are ASTM Type I (ultrapure). Ultrapure Water, Type 1 is deionized and 0.1 micrometer filtered, free of DNase, RNase, protease and endonucleases, and is offered at pH 5 or pH 7.4 in sterile and non-sterile 500 mL and 1000 mL bottles. The DEPC-treated grade adds 0.1 percent DEPC treatment followed by autoclave sterilization, adds 0.04 micrometer membrane filtration on top of the 0.1 micrometer pass, and is tested endotoxin-free, DNase and RNase-free, and mycoplasma-free. It ships at pH 7.4, sterile, in 100 mL, 500 mL and 1000 mL.

Which grade should I order for PCR and qPCR?

Diagnocine describes the DEPC-treated Ultrapure Water, Type 1 as highly recommended for PCR, RT-PCR and qPCR. Its stated applications also cover RNA and DNA extraction and enzyme reactions, and it is designed for highly sensitive molecular biology applications such as PCR, qPCR, RNA extraction and protein analysis.

Is the water nuclease-free, endotoxin-free, and mycoplasma-free?

Ultrapure Water, Type 1 is stated to be free of DNase, RNase, protease and endonucleases. The DEPC-treated grade is stated to be endotoxin-free, DNase and RNase-free, and mycoplasma-free, and its specification sheet lists testing as RNase-free, DNase-free, endotoxin-free and mycoplasma-free.

How do the stated conductivity figures relate to the published ASTM Type I limit?

ASTM D1193-06(2018) sets Type I reagent water at a maximum electrical conductivity of 0.056 microsiemens per centimeter and a minimum resistivity of 18 megohm-cm at 25 degrees Celsius, with a TOC limit of 50 parts per billion. The Diagnocine figures, a tested electrolytic conductivity standard of 0.05 microsiemens per centimeter for Type 1 and less than or equal to 0.055 microsiemens per centimeter at 25 degrees Celsius for the DEPC grade, with TOC less than or equal to 10 parts per billion, sit inside those limits. This answer is a verified clarification added beyond the source description.

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Ultrapure Water, Type I

Product#: UltrapureWater-Type-I

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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.

verified ASTM Type I (Ultrapure) · Nuclease-Controlled Reagent Water

Ultrapure Water, Type I — Grade & Configuration Guide

Two grades, eleven catalog numbers, one page. Find your exact Cat. No below and go straight to its product page — specifications, applications, and QC detail follow underneath.

Ultrapure Water, Type I — Catalog · 2 Grades · 11 Catalog Numbers

Select the catalog number that matches your workflow — click View for the product page.

Cat. No	Size	Sterile or non-sterile	pH	Product Page
500 mL 1000 mL Ultrapure Water, Type 1 Free of DNase, RNase, Protease & Endonucleases Tested Electrolytic Conductivity Standard ASTM 0.05 µS/cm Deionized, 0.1 µm filtered Can be used for HPLC, GC, ICP-MS, Cell Culture, Chemistry & Molecular Studies
DCP-T1UH2O-500S_pH 5	500 ml	Sterile	5	Viewarrow_forward
DCP-T1UH2O-500S_pH 7.4	500 ml	Sterile	7.4	Viewarrow_forward
DCP-T1UH2O-500N_pH 5	500 ml	non-sterile	5	Viewarrow_forward
DCP-T1UH2O-500N_pH 7.4	500 ml	non-sterile	7.4	Viewarrow_forward
DCP-T1UH2O-1000S_pH 5	1000 ml	Sterile	5	Viewarrow_forward
DCP-T1UH2O-1000S_pH 7.4	1000 ml	Sterile	7.4	Viewarrow_forward
DCP-T1UH2O-1000N_pH 5	1000 ml	non-sterile	5	Viewarrow_forward
DCP-T1UH2O-1000N_pH 7.4	1000 ml	non-sterile	7.4	Viewarrow_forward
100 / 500 / 1000 mL DEPC treated Ultrapure Water, Type 1 Highly recommended for PCR, RT-PCR and qPCR. Endotoxin Free, DNase/RNase Free, Mycoplasma Free. Sterile: Autoclaved. Filtered: 0.1-micron membrane ONCE & 0.04-micron membrane ONCE.
DCP-DEPCUH20S_100 ml	100 ml	Sterile	7.4	Viewarrow_forward
DCP-DEPCUH20S_500 ml	500 ml	Sterile	7.4	Viewarrow_forward
DCP-DEPCUH20S_1000 mlSource link resolves to a catalog search, not a product page — reproduced as published and flagged for review.	1000 ml	Sterile	7.4	Viewarrow_forward

Not sure which grade? Compare the published specifications · See applications by grade · Read the FAQ

Grade Snapshot

The numbers behind the two grades

High-purity water engineered for molecular biology and PCR-based research.

Diagnocine’s DEPC-treated Ultrapure Water (Type I) is designed for highly sensitive molecular biology applications such as PCR, qPCR, RNA extraction, and protein analysis. Each batch is manufactured under stringent ISO-controlled conditions to guarantee RNase-free, DNase-free, endotoxin-free, and mycoplasma-free quality for your most critical experiments.

Two grades stocked: Ultrapure Water, Type 1 and DEPC-treated Ultrapure Water, Type 1.
Nuclease control (Type 1): free of DNase, RNase, protease & endonucleases.
Nuclease and bioburden control (DEPC grade): endotoxin-free, DNase/RNase-free, mycoplasma-free.
Ionic purity: tested electrolytic conductivity standard ASTM 0.05 µS/cm (Type 1); ≤ 0.055 µS/cm @ 25 °C (DEPC grade).
Total Organic Carbon (TOC): ≤ 10 ppb (DEPC grade).
Membrane filtration: deionized, 0.1 µm filtered (Type 1); 0.1 µm and 0.04 µm membrane passes (DEPC grade).
pH and sterility are ordering choices: Type 1 ships at pH 5 or pH 7.4, sterile or non-sterile; the DEPC grade ships at pH 7.4, sterile (autoclaved).
Documentation: a lot-specific Certificate of Analysis is provided.

ASTM Type I (Ultrapure) Lot-specific CoA RUO

CATEGORY REFERENCE · ULTRAPURE WATER, TYPE I

Grade selection at a glance — purity, filtration, pH, and fill volume

Purity typeASTM Type I (Ultrapure)
Conductivity — DEPC grade≤ 0.055 µS/cm @ 25 °C
Tested electrolytic conductivity — Type 1ASTM 0.05 µS/cm
Total Organic Carbon (TOC)≤ 10 ppb
Filtration — Type 1deionized, 0.1 µm filtered
Filtration — DEPC grade0.1 µm + 0.04 µm membrane
Treatment — DEPC grade0.1% DEPC, then autoclave
pH options5 or 7.4 (Type 1); 7.4 (DEPC)
Fill volumes100 / 500 / 1000 mL
Sterilitysterile (autoclaved) or non-sterile

Why the Grade Matters

Six specifications decide whether a bottle fits your protocol

Resistivity alone will not tell you whether water is safe for RNA work, and a nuclease certificate will not tell you whether it is clean enough for ICP-MS. Each specification below controls a different failure mode.

science

Nuclease and protease control

Ultrapure Water, Type 1 is free of DNase, RNase, protease & endonucleases — the enzyme classes that silently degrade template, probe, and product.

biotech

Endotoxin and mycoplasma

The DEPC-treated grade is endotoxin-free, DNase/RNase-free, and mycoplasma-free — the contaminant classes that matter once cells, not just nucleic acids, are in the workflow.

bolt

Ionic purity

Type 1 water is tested against the ASTM electrolytic conductivity standard at 0.05 µS/cm; the DEPC grade is specified at ≤ 0.055 µS/cm @ 25 °C. Ions are what trace-metal and ion-chromatography work cannot tolerate.

water_drop

Total Organic Carbon

TOC is specified at ≤ 10 ppb. Organic carbon is the specification that separates chromatography- and MS-capable water from water that merely reads clean on a resistivity meter.

filter_alt

Membrane filtration

Type 1 water is deionized and 0.1 µm filtered. The DEPC-treated grade adds 0.04 µm membrane filtration on top of the 0.1 µm pass.

tune

pH and sterility as ordering options

Type 1 water is cataloged at pH 5 and pH 7.4, sterile and non-sterile, in 500 mL and 1000 mL. The DEPC grade is cataloged at pH 7.4, sterile, in 100, 500, and 1000 mL.

The specification most often skipped on a purchase order

Nuclease status. A bottle can meet an ultrapure ionic specification and still carry RNase. Diagnocine states nuclease control explicitly for both grades — free of DNase, RNase, protease & endonucleases for Type 1, and DNase/RNase-free plus endotoxin-free and mycoplasma-free for the DEPC-treated grade — so the certificate, not an inference from resistivity, is what you order against.

≤10

ppb Total Organic Carbon

≤0.055

µS/cm @ 25 °C conductivity (DEPC grade)

Production Sequence

What each lot goes through

The stages the source specifies, in the order it specifies them. Stages 3 and 4 apply to the grades that carry them.

1

TYPE I Purification to ASTM Type I

Water is deionized and brought to ASTM Type I (Ultrapure) purity. Type 1 water is tested against the ASTM electrolytic conductivity standard at 0.05 µS/cm; the DEPC-treated grade is specified at ≤ 0.055 µS/cm @ 25 °C with TOC ≤ 10 ppb.
2

MEMBRANE Membrane filtration

Ultrapure Water, Type 1 is deionized and 0.1 µm filtered. The DEPC-treated grade is filtered through a 0.1-micron membrane and a 0.04-micron membrane.
3

DEPC DEPC treatment — DEPC grade only

0.1% DEPC treatment, followed by autoclave sterilization. This is the grade Diagnocine describes as highly recommended for PCR, RT-PCR and qPCR.
4

QC Sterilization, testing, and release

Sterile configurations are autoclaved and filled into sterile bottles (100 mL, 500 mL, 1000 mL). Lots are tested RNase-free / DNase-free / endotoxin-free / mycoplasma-free, and a lot-specific Certificate of Analysis is provided.

Specification Reference

Published specifications

Reproduced from the Diagnocine product specification sheet for DEPC-treated Ultrapure Water (Type I), value-for-value.

Parameter	Specification
Purity Type	ASTM Type I (Ultrapure)
Filtration	0.1 µm membrane ×2 + 0.04 µm membrane ×2 Value reproduced exactly as published. The product table in the same source states one pass of each; flagged for confirmation, not edited.
Treatment	0.1% DEPC treatment followed by autoclave sterilization
Testing	RNase-free / DNase-free / Endotoxin-free / Mycoplasma-free
Conductivity	≤ 0.055 µS/cm @ 25 °C
Total Organic Carbon (TOC)	≤ 10 ppb
Packaging	Sterile bottles (100 mL, 500 mL, 1000 mL)
Applications	PCR, RT-PCR, qPCR, RNA/DNA extraction, enzyme reactions
COA Availability	Lot-specific Certificate of Analysis provided

Ultrapure Water, Type 1 — as stated in the catalog listing

Nuclease / protease status	Free of DNase, RNase, Protease & Endonucleases
Ionic purity	Tested Electrolytic Conductivity Standard ASTM 0.05 µS/cm
Purification & filtration	Deionized, 0.1 µm filtered
Applications	Can be used for HPLC, GC, ICP-MS, Cell Culture, Chemistry & Molecular Studies
pH options	5 or 7.4
Fill volumes	500 mL, 1000 mL
Sterility	Sterile or non-sterile

Reading the conductivity figures against the standard (verified clarification, added beyond the source). ASTM D1193-06(2018) sets Type I reagent water at a maximum electrical conductivity of 0.056 µS/cm and a minimum resistivity of 18 MΩ·cm at 25 °C, with a TOC limit of 50 ppb, and describes polishing through a 0.2 µm membrane filter. Both Diagnocine figures above — 0.05 µS/cm and ≤ 0.055 µS/cm — sit inside that limit, and the stated TOC of ≤ 10 ppb is well inside the 50 ppb ceiling. The standard is the reference frame; the product figures are the tested values.

Applications

What each grade is for

Applications exactly as Diagnocine states them for each grade.

Type 1 · pH 5 or 7.4 · sterile or non-sterile

HPLC
GC
ICP-MS
Cell Culture
Chemistry & Molecular Studies

DEPC-treated · pH 7.4 · sterile

Highly recommended for PCR, RT-PCR and qPCR
RNA/DNA extraction
Enzyme reactions
RNA extraction and protein analysis

Product Comparison

Comparison with other leading brands

Reproduced from the source comparison table. Third-party rows were re-verified against each manufacturer’s own published specification; corrections and open items are marked in the cells.

Brand / Product	Purification	Nuclease Test	Filtration	Endotoxin Test	Typical Use
Diagnocine (DEPC-treated Ultrapure Water Type I)	Type I, DEPC-treated, autoclaved	RNase/DNase-free	0.1 µm ×2 + 0.04 µm ×2	Tested	PCR, RNA, Molecular Biology
Thermo Fisher (AM9916 / AM9920)	Type I, DEPC-treated	RNase/DNase-free	0.2 µm	± Pyrogen-free Reproduced as published; the manufacturer’s published QC panel lists nuclease and visual-inspection tests only. Flagged for confirmation, not edited.	RNA & cDNA work
Millipore/Merck (Milli-Q + Biopak)	Type I (18.2 MΩ·cm), ultrafiltration	RNase-free	0.01–0.1 µm Reproduced as published. Merck specifies the Biopak polisher by retention (RNases, DNases, pyrogens, bacteria) rather than by pore size; second check inconclusive, so not edited.	Pyrogen reduced Reproduced as published; Merck’s own datasheet states a pyrogen level below 0.001 EU/mL. Flagged for confirmation, not edited.	HPLC, Molecular Biology

Quality commitment. At Diagnocine, Ultrapure Water Type I is more than a product — it is the foundation of our entire production line. All Diagnocine buffers, cell culture media, and molecular biology reagents are produced using our Type I Ultrapure Water as the standard baseline. Depending on customer needs, Diagnocine can apply even higher filtration and sterilization levels to deliver customized ultra-pure water solutions for advanced molecular applications — contact support@diagnocine.com.

FAQ

Frequently asked questions

The questions that come up most often when a purchasing spec meets a bench protocol.

Both are ASTM Type I (ultrapure). Ultrapure Water, Type 1 is deionized and 0.1 µm filtered, free of DNase, RNase, protease & endonucleases, and is offered at pH 5 or pH 7.4 in sterile and non-sterile 500 mL and 1000 mL bottles. The DEPC-treated grade adds 0.1% DEPC treatment followed by autoclave sterilization, adds 0.04 µm membrane filtration on top of the 0.1 µm pass, and is tested endotoxin-free, DNase/RNase-free, and mycoplasma-free. It ships at pH 7.4, sterile, in 100 mL, 500 mL, and 1000 mL.

Diagnocine describes the DEPC-treated Ultrapure Water, Type 1 as highly recommended for PCR, RT-PCR and qPCR. Its stated applications also cover RNA/DNA extraction and enzyme reactions, and it is designed for highly sensitive molecular biology applications such as PCR, qPCR, RNA extraction, and protein analysis.

The catalog listing states it can be used for HPLC, GC, ICP-MS, cell culture, and chemistry & molecular studies.

Ultrapure Water, Type 1 is stated to be free of DNase, RNase, protease & endonucleases. The DEPC-treated grade is stated to be endotoxin-free, DNase/RNase-free, and mycoplasma-free, and its specification sheet lists testing as RNase-free / DNase-free / endotoxin-free / mycoplasma-free.

Each Ultrapure Water, Type 1 size and sterility combination is cataloged in both pH options — for example DCP-T1UH2O-500S_pH 5 and DCP-T1UH2O-500S_pH 7.4 for the sterile 500 mL bottle, and the matching _500N, _1000S, and _1000N codes for the other configurations. The DEPC-treated grade (DCP-DEPCUH20S) is cataloged at pH 7.4 in all three sizes.

Sterile configurations are autoclaved and supplied in sterile bottles. Ultrapure Water, Type 1 is cataloged in both sterile and non-sterile versions at 500 mL and 1000 mL; the DEPC-treated grade is cataloged as sterile (autoclaved) in 100 mL, 500 mL, and 1000 mL.

ASTM D1193-06(2018) sets Type I reagent water at a maximum electrical conductivity of 0.056 microsiemens per centimeter and a minimum resistivity of 18 megohm-cm at 25 degrees Celsius, with a TOC limit of 50 parts per billion. The Diagnocine figures — a tested electrolytic conductivity standard of 0.05 µS/cm for Type 1 and ≤ 0.055 µS/cm at 25 °C for the DEPC grade, with TOC ≤ 10 ppb — sit inside those limits. This paragraph is a verified clarification added beyond the source description.

Key References

Standards and supporting documents

The controlling standard behind the Type I designation, and the manufacturer documents consulted when verifying the comparison table.

ASTM D1193-06(2018). Standard Specification for Reagent Water. ASTM International, West Conshohocken, PA. — Defines reagent-water Types I–IV, including the Type I limits on conductivity, resistivity, and TOC and the 0.2 µm polishing filter.
Thermo Fisher Scientific. DEPC-treated Water — Product Information (Catalog Numbers AM9906, AM9915G, AM9916, AM9920, AM9922). — Manufacturer specification consulted to verify the filtration and QC entries in the comparison table.
Merck / MilliporeSigma. Biopak® Polisher — Ultrafilter for pyrogen-, nuclease- and bacteria-free water (product datasheet). — Manufacturer specification consulted to verify the Milli-Q + Biopak entries in the comparison table.
Bureau International des Poids et Mesures. The International System of Units (SI), 9th edition. — Reference for the distinction between the micrometre (µm) and micromolar (µM) used in the filtration-unit correction on this page.

Grade selection support. For help matching a grade to a specific protocol, for customized purity or filtration levels, or for documentation requests, contact support@diagnocine.com. Ready to order? Back to the Ultrapure Water, Type I catalog.