SP6 RNA Polymerase, His-tagged
Cat No. FNK-RP-SPRP-S
Quantity: 500KU
Description
SP6 RNA Polymerase, His-tagged is a DNA-dependent RNA polymerase used for in vitro transcription[1][3]. Here are some key points about this enzyme:
1. Function: It catalyzes the synthesis of RNA from DNA templates containing the SP6 promoter sequence[3].
2. Specificity: SP6 RNA Polymerase is highly specific for the SP6 phage promoter[3].
3. Applications:
- In vitro mRNA synthesis
- Generation of RNA probes
- Production of RNA for various research and biotechnology applications[1][3]
4. Reaction conditions:
- Typically uses a 1X RNA Polymerase Reaction Buffer
- Requires ribonucleotide triphosphates (ATP, UTP, GTP, CTP)
- Incubation at 37°C[3]
5. Features:
- Can incorporate modified nucleotides (e.g., radioactive or fluorescently labeled)[1]
- His-tagged version allows for easier purification[2]
6. Source: Recombinantly produced in E. coli[1]
7. Storage: Usually stored in a buffer containing glycerol and other stabilizing agents[1]
The His-tagged version of SP6 RNA Polymerase offers the advantage of simplified purification using affinity chromatography, which can be beneficial for certain experimental setups or large-scale production of the enzyme[2].
Citations:
[1] https://www.promega.com/products/cloning-and-dna-markers/molecular-biology-enzymes-and-reagents/sp6-rna-polymerase/
[2] https://diagnocine.com/Product/SP6-RNA-Polymerase-Histagged/94014
[3] https://www.neb.com/en-us/products/m0207-sp6-rna-polymerase
[4] https://www.takarabio.com/products/mrna-and-cdna-synthesis/in-vitro-transcription/rna-polymerases-and-rntps/sp6-rna-polymerase
[5] https://www.thermofisher.com/order/catalog/product/EP0131
