Tris-HCl with SDS Buffer
Cat No.: DCP-THSDS3X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
The Tris-HCl with SDS Buffer is a specialized buffer used in the Tricine-SDS-PAGE system, particularly for separating proteins and peptides in the range of 1-100 kDa. This buffer plays a crucial role in gel casting for this electrophoresis technique, which is optimized for resolving small proteins and peptides. The high concentration of Tris-HCl (3 M) and the specific pH of 8.45 create optimal conditions for protein separation in this system. The inclusion of 0.3% SDS ensures protein denaturation and imparts a uniform negative charge to the proteins, facilitating their separation based on molecular weight. This buffer is distinct from typical gel loading or running buffers, as it lacks components like glycerol or tracking dyes, and has a much higher Tris concentration.
Advantages:
- Improved Resolution of Small Proteins and Peptides: Allows separation of proteins in the range of 1-100 kDa, with optimal resolution for proteins as small as 2 kDa.
- Enhanced Separation and Clarity: Minimizes interference from SDS micelles, resulting in sharper bands and higher resolution for smaller proteins.
- Improved Electrophoresis Conditions: Operates at a lower pH compared to Tris-Glycine systems, which helps minimize protein modifications.
Application:
- Separation of Small Proteins and Peptides: Optimal for separating proteins in the mass range of 1-100 kDa, with exceptional resolution for proteins smaller than 30 kDa.
- Gel Casting: Used specifically for preparing gels in the Tricine-SDS-PAGE system.
- Analysis of Hydrophobic Proteins: Facilitates easier transfer of hydrophobic proteins during Western blotting due to lower acrylamide concentrations.
Composition:
| Ingredients |
Concentration |
| Tris-HCl |
3 M |
| SDS |
0.3% |
Quality Control:
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Appearance: Clear
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pH: 8.45
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
