Transferase

Product#: Transferase

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Image	Cat.No:	Size	Storage	Link
	SB-G3412-50UL	50UL	Transport in wet ice packs; store at -20°C, valid for 12 months.
	SB-G3455-2000U	2000U	Transport with wet ice; Store at -20℃, valid for 12 months.

Precautions for SB-G3412-50UL

Enzyme products should be placed in an ice box or on an ice bath when used, and stored at -20°C immediately after use.
Since 5×Reaction Buffer contains CoCl 2 , it is not compatible with downstream applications. The reaction mixture needs to be purified by column centrifugation or phenol/LF extraction and ethanol precipitation to remove CoCl 2 .
For your safety and health, please wear a lab coat and disposable gloves for operation.

Precautions for SB-G3455-2000U

Involving RNA operation, it is necessary to strictly follow the specifications of RNA operation to avoid RNase contamination. Relevant reagents and consumables need to be treated with DEPC to remove RNase or ensure that they are RNase free.
Reaction heat treatment of RNA is used to remove the secondary structure at the 5' end. If the structure of the 5' end is complex, the time can be extended to 10 min.
If the capping effect is not good, the reaction time can be extended to 1 h to improve the capping efficiency.
SAM is unstable at pH 7-8 and 37°C, and needs to be freshly prepared before the reaction. Dilute 32mM SAM to 4 mM working solution before starting the reaction. To avoid SAM degradation, the whole process needs to be placed on ice.
It is recommended to apply RNase inhibitors to prevent RNA degradation during the reaction. An RNase inhibitor ( G3414 ) can be added at a final concentration of 1 U/μL .
For your safety and health, please wear a lab coat and disposable gloves for operation.