Spinner Balanced Salts, Eagle w/ Phenol red and Sodium bicarbonate w/o Calcium chloride 1X (Liquid)
Cat No.: DCP-SBSRB-C1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Spinner Balanced Salts, Eagle w/ Phenol red and Sodium bicarbonate w/o Calcium chloride 1X (Liquid) is a cell culture medium optimized for suspension cell growth in spinner flasks. It is derived from Eagle's Minimum Essential Medium (EMEM) with Hanks' balanced salt solution (HBSS) modifications but excludes calcium chloride (CaCl₂) and magnesium sulfate (MgSO₄) to prevent cell clumping or adhesion. Omited calcium chloride to avoid cell aggregation makes it ideal for suspension-adapted cells like hybridomas or HeLa derivatives.
Applications:
- Vaccine Production: Suitable for adenovirus, influenza, and other viral vector cultures.
- Monoclonal Antibody Development: Supports hybridoma cell proliferation.
- Bioreactor Compatibility: Optimized for high-density cell growth in large-scale spinner flasks.
Advantages:
- Calcium chloride exclusion: Prevents cell aggregation or adhesion, ideal for hybridomas, HeLa derivatives, and other suspension-adapted cell lines.
- Ionic balance: Adjusted magnesium and potassium levels support high-density cell proliferation in spinner flasks or bioreactors.
Composition:
| Ingredients |
mg/L |
| INORGANIC SALTS |
|
| Magnesium sulfate anhydrous |
100.00 |
| Potassium chloride |
400.000 |
| Sodium bicarbonate |
2200.000 |
| Sodium chloride |
6800.000 |
| Sodium dihydrogen phosphate anhydrous |
1220.000 |
| OTHERS |
|
| D-Glucose |
1000.000 |
| Phenol red sodium salt |
11.000 |
Quality Control:
- Appearance: Red colored, clear solution.
- pH: 7.2
- Osmolality in mOsm/Kg H2O: 270.00 - 310.00
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 15-30 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
