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Rat Interleukin 1β,IL-1β ELISA Kit
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Rat Interleukin 1β,IL-1β ELISA Kit
Sandwich quantitative immunoassay for Rat Interleukin 1β,IL-1β in rat serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Uniprot No. | Q63264 |
| Species | Rattus norvegicus (Rat) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 31.25 pg/mL-2000 pg/mL |
| Sensitivity | 15.6 pg/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Immunology |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Rat Interleukin 1β,IL-1β in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 31.25 pg/mL-2000 pg/mL with a minimum detectable dose of 15.6 pg/mL.
This ELISA kit provides quantitative measurement of interleukin-1β (IL-1β) in rat samples, supporting research into innate immune signaling, neuroinflammation, and inflammatory disease models. Because IL-1β is a rapidly induced, tightly regulated cytokine whose circulating levels can vary dramatically between baseline and acute inflammatory states, selecting an assay with appropriate range and sensitivity is critical for accurate quantification across experimental conditions.
Detection Range: The 31.25–2000 pg/mL range spans from near-basal rat serum IL-1β concentrations through the elevated levels observed in LPS challenge, adjuvant-induced arthritis, and traumatic brain injury models, minimizing the need for serial dilutions or sample concentration in most in vivo paradigms.
STRING: 10116.ENSRNOP00000006308
UniGene: Rn.9869
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
2000 | 2.826 | 2.634 | 2.730 | 2.569 | |||
1000 | 2.045 | 1.967 | 2.006 | 1.845 | |||
500 | 1.349 | 1.328 | 1.339 | 1.177 | |||
250 | 0.818 | 0.829 | 0.824 | 0.662 | |||
125 | 0.474 | 0.491 | 0.482 | 0.321 | |||
62.5 | 0.344 | 0.353 | 0.349 | 0.187 | |||
31.25 | 0.238 | 0.244 | 0.241 | 0.080 | |||
0 | 0.157 | 0.165 | 0.161 |
| |||
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 91 | ||||
Range % | 91-105 | |||||
1:2 | Average % | 102 | ||||
Range % | 95-109 | |||||
1:4 | Average % | 99 | ||||
Range % | 92-105 | |||||
1:8 | Average % | 93 | ||||
Range % | 86-98 | |||||
The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 85-98 | ||||
EDTA plasma (n=4) | 97 | 90-100 | ||||
