Protease Inhibitor Cocktail with PBS NP-40 Buffer
Cat. No. DCP-PICPNP1X
Size: 10x10 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
This Ready-to-use combination of a protease inhibitor cocktail with PBS NP-40 Buffer is a comprehensive solution designed for efficient cell lysis and protein extraction while preserving protein integrity. The inclusion of NP-40 facilitates cell membrane disruption, while the PBS maintains a physiological pH and osmolarity. This versatile solution is ideal for various applications in protein research, including Western blotting, immunoprecipitation, and enzymatic assays, offering a convenient and effective means to preserve protein samples and ensure reliable experimental results.
The cocktail contains AEBSF.HCl, a serine protease inhibitor; Aprotinin, which targets serine proteases and has anti-inflammatory properties; Bestatin, an aminopeptidase inhibitor; E-64, a cysteine protease inhibitor; EDTA, a metalloproteinase inhibitor; Leupeptin, which inhibits both serine and cysteine proteases; and Pepstatin A, an aspartic protease inhibitor. This cocktail effectively minimizes protein degradation in a wide range of experimental procedures, including cell lysis, protein purification, and enzymatic assays. The carefully balanced concentrations of each component ensure optimal inhibition of proteolytic activity while maintaining compatibility with most downstream applications, making it an invaluable tool for researchers working with sensitive protein samples.
Advantages:
- Maintains protein integrity during extraction
- Preserves many protein-protein interactions
- Suitable for various downstream applications
- The combination of PBS and NP-40 provides a stable environment that maintains physiological pH and osmolarity, crucial for preserving protein structure during extraction.
Application:
- Western Blotting: Preserves protein samples for accurate analysis.
- Cell Lysis: The buffer is used to lyse cells while protecting proteins from degradation.
- ELISA and Other Immunoassays: Preserves antigenic epitopes for detection.
- Protease Inhibition Studies: Used in screening potential protease inhibitors.
- Cell Culture Experiments: Protects proteins in various cell culture applications.
Composition:
| Ingredients |
Concentration |
| AEBSF.HCl |
100 uM |
| Aprotinin |
0.8 uM |
| Bestatin |
0.05 mM |
| E-64 |
1 uM |
| EDTA |
5 mM |
| Leupeptin |
0.01 mM |
| Pepstatin A |
1 uM |
| NP-40 |
1% |
| Potassium Chloride |
2.7 mM |
| Sodium Chloride |
137 mM |
| Sodium Phosphate dibasic |
10 mM |
| Potassium Phosphate monobasic |
1.8 mM |
Quality Control:
-
Appearance: Clear solution.
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
-
DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -80 ℃
-
Shelf life: 3 months
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
