Protease Inhibitor Cocktail with EDTA (100X)
Cat. No. DCP-PICEDTA100X
Size: 1 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
This comprehensive 100x protease inhibitor cocktail is a potent blend designed to provide broad-spectrum protection against various classes of proteases in biochemical and cellular applications. The cocktail contains AEBSF.HCl, a serine protease inhibitor; Aprotinin, which targets serine proteases and has anti-inflammatory properties; Bestatin, an aminopeptidase inhibitor; E-64, a cysteine protease inhibitor; EDTA, a metalloproteinase inhibitor; Leupeptin, which inhibits both serine and cysteine proteases; and Pepstatin A, an aspartic protease inhibitor. When diluted 100-fold to working concentration, this cocktail effectively minimizes protein degradation in a wide range of experimental procedures, including cell lysis, protein purification, and enzymatic assays. The carefully balanced concentrations of each component ensure optimal inhibition of proteolytic activity while maintaining compatibility with most downstream applications, making it an invaluable tool for researchers working with sensitive protein samples.
Advantages:
- Broad-spectrum protection against various classes of proteases (serine, cysteine, aspartic, and aminopeptidases).
- Versatile application in multiple research procedures (cell lysis, protein purification, enzymatic assays).
- Improved protein stability by inhibiting proteolytic degradation.
- Optimized formulation with carefully balanced concentrations of each inhibitor.
- Enhanced compatibility with downstream applications.
Application:
- Protein purification and extraction.
- Cell lysis procedures.
- Protein expression and characterization studies.
- Western blotting.
- Cell culture experiments
Composition:
| Ingredients |
Concentration |
| AEBSF.HCl |
10 mM |
| Aprotinin |
80 uM |
| Bestatin |
5 mM |
| E-64 |
100 uM |
| EDTA |
0.5 M |
| Leupeptin |
1 mM |
| Pepstatin A |
0.1 mM |
Quality Control:
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Appearance: Clear solution.
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
