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OptiPreserv

Product#: DCP-OPCM1X
$1.00
DCP-OPCM1X
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OptiPreserv

Cat. No. DCP-OPCM1X
Sizes: 50 ml and 5 ml (trial)

Description

OptiPreserv represents a breakthrough in stem cell cryopreservation technology—a protein-free freezing medium designed to deliver exceptional cell protection and recovery. Built on the latest advances in cryobiology, OptiPreserv offers superior cell protection in a optimized formulation.

This chemically defined medium eliminates the need for DMSO, animal-derived proteins, and undefined components while delivering exceptional post-thaw viability and maintaining pluripotency markers across multiple stem cell types.

Key Features & Benefits

Advanced Cryoprotection Technology

  • Optimized cryoprotectant system: Ethylene glycol (10% v/v) as primary permeating agent

  • Ice recrystallization inhibition: Polyvinyl alcohol (PVA) + Poly(methyl vinyl ether-alt-maleic acid)

  • Membrane stabilization: Trehalose (0.1M) and sucrose (0.1M)

  • Extracellular protection: Dextran 40 (5% w/v) for osmotic stabilization

Enhanced Antioxidant Protection

  • Dual antioxidant system: L-ascorbic acid (1g/L) + reduced glutathione (3mM)

  • Calcium chelation: EGTA (1mM) prevents calcium-induced damage

  • Osmoprotection: L-proline (0.1M) provides additional cellular protection

Quality

  • DMSO-free - eliminates cytotoxicity concerns for therapeutic applications

  • Protein-free - removes batch-to-batch variation and animal-derived components

  • Xeno-free - meets stringent requirements for human therapeutics

  • Serum-free - ensures consistent, defined composition

Applications

Research Applications

  • Induced pluripotent stem cells (iPSCs)

  • Embryonic stem cells (ESCs)

  • Mesenchymal stem cells (MSCs)

  • Hematopoietic stem cells

  • Neural stem cells

  • Primary cell cultures

Clinical & Therapeutic Applications

  • Cell therapy manufacturing

  • Biobanking and storage

  • Drug discovery programs

  • Regenerative medicine research

  • Toxicology screening

Specialized Applications

  • Long-term cell line maintenance

  • Large-scale bioprocessing

  • Quality control studies

  • Method development and validation

Scientific Foundation

Mechanism of Action

Optipreserv employs a multi-modal approach to cellular cryoprotection:

  1. Permeating Cryoprotection: Ethylene glycol penetrates cells to prevent intracellular ice formation

  2. Membrane Stabilization: Trehalose interacts with phospholipid membranes to maintain structural integrity

  3. Ice Recrystallization Inhibition: PVA and Poly(methyl vinyl ether-alt-maleic acid) prevent damaging ice crystal growth during thaw

  4. Extracellular Protection: Dextran 40 maintains osmotic balance and prevents cell dehydration

  5. Antioxidant Defense: Dual antioxidant system neutralizes reactive oxygen species

  6. Calcium Management: EGTA chelation prevents calcium-mediated cellular damage

Technical Specifications

Component Function Concentration
Ethylene glycol Permeating cryoprotectant   10% v/v
Trehalose Membrane stabilizer    0.1 M
Polyvinyl alcohol Ice recrystallization inhibitor    1% w/v
Poly(methyl vinyl ether-alt-maleic acid) Macromolecular cryoprotectant    2% w/v
Dextran 40 Extracellular protectant    5% w/v
L-Glutathione (reduced) Antioxidant    3 mM
L-Ascorbic acid Antioxidant    1 g/L
EGTA Calcium chelator    1 mM
L-Proline Osmoprotectant    0.1 M
Sucrose  Membrane stabilizer    0.1 M

Physical Properties

  • pH: 7.4

  • Sterility: 0.1 μm filtered, endotoxin-free

  • Appearance: Clear, colorless solution

  • Storage: 2-8°C, -20°C (extended storage). Working storage: Room temperature for up to 4 hours once opened.

Protocol & Usage

Standard Freezing Protocol

  1. Cell Preparation: Harvest cells at 70-80% confluence

  2. Medium Addition: Resuspend cells 1:1 with OptiPresev

  3. Cooling Rate: 1°C/minute using controlled rate freezer or isopropanol bath

  4. Storage: Transfer to liquid nitrogen (-196°C) after reaching -80°C

  5. Thawing: Rapid thaw in 37°C water bath, dilute immediately

Optimization Guidelines

  • Cell density: 1-5 × 106 cells/mL recommended

  • Cooling rate: Adjustable for different cell types

  • Storage duration: Validated for >2 years at -196°C

  • Thaw protocol: Cell-type specific modifications available

Handling Guidelines

  • Maintain sterile aseptic technique throughout use

  • Allow to equilibrate to room temperature before use

  • Mix gently by inversion before aliquoting

  • Use appropriate personal protective equipment

  • Dispose according to institutional guidelines

* All DiagnoCine Precision Sterile media are filtered-sterilized with a 0.1-micron filtration two times and 0.04 micron once. Thus, mycoplasma contamination is prevented.  The smallest size mycoplasma type can be about 0.2 microns.

* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center.  All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA 

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