HydroCell (Flask)

Quick Link	Products	Cat. No.	Packing Unit (pcs)	Size (mm)	Surface Area	Capacity per well	Link
HydroCell (Flask)	T25 Flask	FNK-CSF025	12	81(W)×125(L)	25 cm2	70 mL
	T75 Flask	FNK-CS2015	5	40(D)×12(H)	8.8 cm2	3 mL

Hydrocell is a low cell binding cultureware. On its surface, super hydrophilic polymer was immobilized at nano-thickness.
This hydrophilic character helps the formation of embryoid bodies of ES/iPS cell or Spheroid in culture.
Compared to other brands' low cell binding cultureware, HydroCell shows lower cell binding.

Features

• Super hydrophilic polymer is immobilized to the surface of the cultureware at nano-thickness.
• Lowest attachment rate among similar competitive brands.
• Three formats : Microplates, Dishes and Flask types.

Best for...

• Formation of embryoid bodies of ES cells in culture
• Formation of anchorage-independent colonies
• Differentiation such as cartilage (spheroid formation)
• Culture & storage of macrophage/immune cells
• Screening of anti-cancer drugs (replacement for soft agar assays)

Image of HydroCell surface Super hydrophilic polymer is immobilized to the surface of the cultureware at nano-thickness. Suitable for Formation of embryoid bodies of ES/iPS cells in culture Spheroid formation in culture Replacement for soft agar assays Screening for anti-cancer drugs.

Macrophages from mouse peritoneum in medium with serum were incubated for 3 days in (1) cultureware with HydroCell (2) another brand of low cell binding cultureware, and (3) non-treated cell cultureware. Only the HydroCell allowed uniform suspension culture (more than 99% of the cells remained in suspension) and simple cell dissociation and harvesting by pipetting.

Nomal Rat Kidney (NRK) cells in medium with serum were seeded in cultureware with HydroCell or in a low cell binding product of brand A, B or C. Degree of cell adhesion was determined using a TetraColor ONE assay system (Seikagaku Corp.; Tokyo, Japan) at seeding and after 5 days of incubation.

Left : Uniform suspension culture with HydroCell Right : Cell adhesion on bacterial petri dish *Cultured in medium with 20% FBS

Murine ES cells (B6G-2) were incubated for 2 days in HydroCell 96MW     Photos taken on slide glass

Noise produced by growth of non-cancer cells mixed with cancer cells can be inhibited

Protocol : 1. The following cells were seeded in HydroCell 96MW and non-treated cell cultureware group I : A-549 (2.0 X 103 cells) group II : Human Dermal Fibroblasts (HDF, 2.0 X 103 cells) group III : A-549 (2.0 X 103 cells) + Human Dermal Fibroblasts (HDF, 2.0 X 103 cells) 2. Genistein, concentration range 0 - 100ug / mL, was added to each well and incubated for 3 days. 3. 10uL of live cell counting reagent was added to each well, and the absorbance OD450 was measured after 4 hours.

	(A) hiPS Cells Sphere Formation iPS spheres are formed on HydroCell (left photo) compared to the adherence to the edge of other brand dish (right photo) <A. Umezawa, National Center for Child Health and Development>
	(B) hES Cells Sphere Formation Method A short time floating culture is extremely useful as a way to sphere formation in the induction of differentiation on feeder-free conditions. Step1: hES cells maintained without feeder cells Step2: Sphere formation on HydroCell Step3: Adherent culture of spheres on a gelatin-coated dish <Kumiko Saeki, International Medical Center of Japan, Tokyo, Japan>