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Human mammary carcinoma marker CA15-3 (CA15-3) ELISA kit
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Human mammary carcinoma marker CA15-3 (CA15-3) ELISA kit
Sandwich quantitative immunoassay for Human mammary carcinoma marker CA15-3 (CA15-3) ELISA kit in human serum available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Uniprot No. | P15941 |
| Species | Homo sapiens (Human) |
| Sample types | serum |
| Detection range | 15 U/mL - 250 U/mL |
| Sensitivity | 3.8 U/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cancer |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Human mammary carcinoma marker CA15-3 (CA15-3) ELISA kit in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 15 U/mL - 250 U/mL with a minimum detectable dose of 3.8 U/mL.
This Human CA15-3 ELISA Kit was designed for the quantitative measurement of Human CA15-3 protein in serum. It is a Sandwich ELISA kit, its detection range is 15 U/mL - 250 U/mL and the sensitivity is 3.8 U/mL.
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- We have analysed the tumour-associated carbohydrate antigens sialyl-Lewis x (SLe(x)) and sialyl-Tn (STn) on MUC1 and MUC5AC in Pancreatic adenocarcinoma (PDAC)tissues. immunoprecipitation of MUC5AC from positive PDAC tissues and subsequent SLe(x) immunodetection confirmed the presence of SLe(x) on MUC5AC. Altogether, MUC5AC-SLe(x) glycoform is present in PDAC and can be regarded as potential biomarker. PMID: 29408556
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- MUC1 was a potential molecular target may help explain the role of lincRNA-ROR/miR-145 for invasion and metastasis in Triple-negative breast cancer cell lines. PMID: 29673594
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- High MUC1 expression is associated with cervical cancer. PMID: 30062487
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<20% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| |||||||
U/ml | OD1 | OD2 | Average |
| |||
0 | 0.109 | 0.114 | 0.112 |
| |||
15 | 0.187 | 0.196 | 0.192 |
| |||
40 | 0.425 | 0.401 | 0.413 |
| |||
100 | 0.964 | 0.975 | 0.970 |
| |||
250 | 1.937 | 1.836 | 1.887 | ||||
To assess the linearity of the assay, samples were spiked with high concentrations of human CA15-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 90 | ||||
Range % | 86-99 | |||||
1:2 | Average % | 97 | ||||
Range % | 91-105 | |||||
1:4 | Average % | 95 | ||||
Range % | 89-100 | |||||
1:8 | Average % | 92 | ||||
Range % | 88-98 | |||||
The recovery of human CA15-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 97 | 93-99 | ||||
EDTA plasma (n=4) | 96 | 90-101 | ||||
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CA15-3 antibody.
- Five vials standard (0.5 ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated CA15-3 antibody (6 ml/bottle) --- Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.
- One vial Sample Diluent (11ml/bottle) ---Dilute the sample solution.
- One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.
- One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.
- One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.
- One vial Stop Solution (7ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at603 nm or 630 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
