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Human Immunoglobulin E,IgE ELISA Kit
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All-inclusive format covers everything from sample preparation to signal detection. Every step can be customized and optimized for your precision research.
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* all types of species
* HRP and AP detection
* Low target signal enhancement
* Inquire about a trial version or a customized version
4 core reagents below are included with every order, plus 1 of the 2 detection-specific reagents (matched automatically to your kit type) — 5 components, every time.
+ the 5th component, matched to your kit's detection method:
If you have any questions or need additional information, please call us at 551-245-1033 or email us at support@diagnocine.com. Our team will be happy to assist you.
Universal Compatibility
All buffers and detection reagents work seamlessly across target proteins, bridging the gap in assay consistency.
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Enhanced Precision
Designed by scientists for scientists, all components are engineered to deliver sharper results and an optical signal.
Human Immunoglobulin E,IgE ELISA Kit
Sandwich quantitative immunoassay for Immunoglobulin E,IgE in human serum, plasma available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | Immunoglobulin E,IgE |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma |
| Detection range | 62.5 ng/mL-4000 ng/mL |
| Sensitivity | 3.640 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Immunology |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Immunoglobulin E,IgE in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 62.5 ng/mL-4000 ng/mL with a minimum detectable dose of 3.640 ng/mL.
This Human IgE ELISA Kit was designed for the quantitative measurement of Human IgE protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 62.5 ng/mL-4000 ng/mL and the sensitivity is 3.640 ng/mL.
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
To assess the linearity of the assay, samples were spiked with high concentrations of human IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
