Glucose 1-Dehydrogenase, Thermostable

Glucose 1-Dehydrogenase, Thermostable

Cat. No.  FNK-GCD-41-01
Stroage  -20°C
Size  500 units 
Volume 1 ml
EC No.  1.1.1.47
Substrate  glucose
Reaction product gluconolactone
Reaction temperature  ~70℃
Reaction pH   5.0~8.0
Lot No. 1-H202
Protein conc10 mg/ml
Form  50 mM Tris-HCl (pH 8.0)
Activity 546 U/ml
Notes For research use only.

Description

EC No.:1.1.1.47 Substrate: glucose Reaction product: gluconolactone Reaction temperature : ~70℃ Reaction pH : 5.0~8.0
Enzymes work at high temperature with high reactivity The "Thermostable Enzyme".

Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.

Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.

Features of Thermostable Enzyme

• Highly stable and durable for long term use
• High reactivity at high temperature (70 - 100°C)
• Resistance to organic solvent is expected
• Special instruments to inhibit bacterial growth are not required.
• Reaction speficity is much higher compared with chemical reaction
• Speed of reaction can be accelerated

Activity measurement

Reaction mix (50 mM PIPES-KOH (pH 6.5), 10 mM Glucose, 1 mM NADP+ and appropriate amount of the enzyme) was incubated at 37 oC and A340 was monitored. One unit is defined as the amount of the enzyme producing 1 µmol of NADPH (e340=6.22 mM-1 cm-1) per 1 minute using Glucose as a substrate.