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DynaMarker® Protein MultiColor Stable II (Trial) (Only 1 Per Customer)

Product#: FNK-DM660-Trial

$1.05

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DynaMarker® Protein MultiColor Stable II (*Only 1 Per Customer*)

Cat. No. FNK-DM660-Trial
Size Trial
Storage 4 °C
Stability 12 months at 4 °C
Storage Buffer Tris-HCl (pH6.8), EDTA, Glycerol, SDS

Product ID and Size

Cat. No.	Size	Link
FNK-DM660-X1	1.2 ml
FNK-DM660L-X5	5 x 1.2 ml
FNK-DM660-Trial	Trial

Protein MultiColor Stable Ⅱ
*This is a renewal product of Protein MultiColor Ladder Marker, Stable.

Renewal Points !

*The product name has changed from "Stable" to "StableⅡ".
*Aprotinin (Green) of 8.5kDa has been newly added.
*The loading amount per one lane has changed from 5ul to 10ul. The number of loads is still the same (120 times).

Description

Pre-stained protein ladder for broad range of proteins (8 to 230 kDa), stable in refrigerator ( at 4℃ ) for 1 year!

The DynaMarker® Protein MultiColor Stable II is a pre-stained protein molecular weight marker. The marker has a remarkable feature that it is possible to store at 4 °C. The feature allow us to start electrophoresis with the marker, because it is always in a liquid state while stored at 4 °C. The DynaMarker® Protein MultiColor Stable II consists of nine prestained proteins. Each of them are stained red, blue, purple, green or orange, ranging in apparent molecular weight from approximately 8 kDa to 230 kDa. The DynaMarker® Protein MultiColor Stable II is suitable for visualizing proteins during electrophoresis without staining and for monitoring electrophoretic transfer onto membranes. The protein concentrations are optimized to give uniform band intensities. The marker is supplied in gel loading buffer for direct loading onto SDS-PAGE without heating or adding reducing agents.

Electrophoresis profile of DynaMarker Protein MultiColor Stable II (10 μl) on 6% polyacrylamide (5% C) Gel / AllView PAGE Buffer (Code#DS520) as running buffer.

Feature

Multicolored pre-stained natural proteins
Stable for up to 1 year at 4℃
Ready-to-load (10μL / lane)
Sharp bands
M.W. range from approx. 8 to 230 kDa

Applications

Real-time monitoring of protein mobility on SDS-PAGE gel.
Can check transfer efficiency on membranes without staining.

Protocol

Take the marker out of refrigerator.
Load 10 μl for mini-gels or more for large size gels.
Load your samples.
Start electrophoresis.

Note There is no need to heat or add reducing agents

Contents

Apparent molecular weight (kDa) *

Protein	Tris-Glycine-SDS buffer (Laemmli running buffer)	AllView PAGE Buffer (Code#DS520)
Myosin	249	250
β-Galactosidase	137	141
Phosphorylase-b	96	100
BSA	73	71
Ovalbumin	46	45
Carbonic anhydrase	31	30
Soybean trypsin inhibitor	26	25
Lysozyme	18	17
Aprotinin	6.3	8.4

Note

As covalently bound dye affects protein mobility, each batch of prestained protein marker is calibrated against
unstained standards. A prestained protein marker should be used for approximate molecular weight determination. For
precise molecular weight determination use an unstained molecular weight marker.

* : The apparent molecular weight values are lot specific and depends on the electrophoresis running buffer.

Comparison

Supplier	BioDynamics Laboratory (BDL)	Company A	Company B	Company C	Company D
Number of loads	120	50	50	50	50
Storage Temp.	4℃	-20℃	-20℃	4℃	4℃
Shelf Life	1 year	3 months	1 year	4 months	3 months

Related product

Code	Product Name	Description
DS520	All View PAGEBuffer	AllView PAGE Buffer is a new type of running buffer for SDS-PAGE electrophoresis. This buffer is possible to separate proteins with wide range of molecular weights in the basic Laemmli gel (Tris-HCl) similar to using “gradient gel”.