Disulfide Bond Enhancer Enzyme Set
Disulfide Bond Enhancer Enzyme Kit
Cat. No.: FNK-CFS-DBEE
Size: 1 Kit
This Product has a shelf life of 1 year being safely stored at -80°C.
CFS products are for research use only.
Description:
This product does not contain any reagents to perform transcription and translation experiments.
It was designed to modify the reaction conditions for better formation of disulfide bonds when working with the WEPRO7240H Core Kit. Reaction conditions are modify by adding a human endoplasmic reticulum oxidoreductase (Ero1α) and a Protein disulfide isomerase (PDI) to the translation reaction while using a different buffer composition for bilayer formation.
Do not use the WEPRO7240 and WEPRO7240G Core kits as this will reduce protein yields. The GST-tag is unsuited when working with buffers containing glutathione as this will lead to a loss of disulfide bonds.
Confirm reaction conditions using the Disulfide Bond PLUS Expression Kit.
Features:
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In combination with the WEPRO7240H Core Kit, this set allows to prepare proteins on different scales using the bilayer format
- Users who want to prepare proteins with disulfide bonds
Flow of operations:
