Extrap Soil DNA Kit Plus Ver.2

Cat.No: FNK-212-006

Size: 50 preps (1 kit)

Storage: Room Temperature, *This product should be stored at room temperature and protected from light (10-30℃). After opening, store at 2-4℃. *Do not open the cap of bread tube before use. 

Shelf life: 1 year
 

Features

Extrap Soil DNA kit Plus ver.2 is a kit for extraction and purification of microbial DNA from environmental samples such as soil and activated sludge. This product is suitable for applications such as microbial community structure analysis in environmental samples and real-time PCR. The kit provides high recovery and high purity of DNA from a wide range of enivronmental samples.

* Purifying DNA from a wide range of microbial species via bead beating disruption.

* Our proprietary technology that inhibits the adsorption of DNA on soil particles, enabling high recovery of DNA from environmental samples.

* Easy DNA purification with magnetic beads and applicable for automation.

* Organic solvents such as phenol or chloroform are not required.
 

Key Contents

Componets	Volumes
Bead Tubes	50 tubes
Extraction Buffer	71 mL
Lysis Solution	4 mL
PP Solution	18 mL
MBs Solution	3 mL
Binding Solution	54 mL
Washing Solution	48 mL

*Lysis solution, Binding solution, and Washing solution may percipitate at low temperatures. In that case, warm the bottle at 45-60℃ to completely dissolve the percipitate. 

*Water droplets may be seen inside the Bead Tubes, but this does not indicate a quality problem. 
 

Required materials not included with the kit

1. Reagents

* 70% ethanol

* Elution buffer (TE buffer, sterile water, etc.)

2. Equipment / Instruments

* Micropipettes and tips

*1.5 mL tubes

* 2 mL tubes

* Magnetic rack for 1.5 mL

• Recommended product: Magtrap<Magnetic separator> (BDL, Cat No. DD100)

* Cell disruptor (for 2 mL tubes)

• Recommended products: FastPrep series (MP Biomedicals), Bead Crusher μT-01 (TAITEC)

* Vortex Mixer

* Centrifuge (>14,000 x g)

* Heat block or water bath
 

Caution

* When using this product, general laboratory precautions should be taken, and safety precautions should be followed.

* This product is a research use only. Do not use this product for diagnostics or any other purpose.

* BioDynamics Laboratory Inc. is not responsible for any problems caused by using the product in a manner different from that described in the instruction manual.

* Wear appropriate protective equipment.

* Do not use damaged bottles or contaminated solutions.

* Do not add additional inhibitors to the product. This product already contains DNA adsorption inhibitors.

* The contamination of this kit is less than 10 copies of 16S rDNA/μL of DNA extract.

* Do not open the cap of bead tube before use.

* Ensure that the O-ring on the cap is in place before use.

* The DNA binding capacity of magnetic beads is 10 to 20 µg /tube.