Disposable Hemocytometer (*Only 1 Per Customer*)

Product Name	Cat. No.	Size	Storage	Link
Disposable hemocytometer (4-chambers), 20 pcs	FNK-521-10-S	20 pcs	RT
Disposable hemocytometer 50pcs	FNK-521-10-1	50 pcs	RT
Disposable hemocytometer (4-chambers) 200pcs	FNK-521-10-L	200 pcs	RT
Disposable hemocytometer (Trial)	FNK-521-10-Trial	1 Pcs	RT

Description
 

4 multi cell type Disposable Cell Counting Chamber made of plastic.

• Designed for rapid loading
• No washing, no labor, save time
• Same grid as standard one
• High calculation accuracy
• Made of durable and high grade plastic

Features of Disposable Hemocytometer
 

• Cost effective : 4 chambers for 1 slide.
• Designed for easyloading
• Disposable : No wash, save time - good for infectious sample
• Same grid as standard hemocytometer
• High calculation accuracy
• Made of durable plastic
• Great correlation result with Neubauer standard hemocytometer.  

Sample Preparation
 

1. Prepare cell suspension. When counting adherent cells, disperse cells by cell detachment solution such as Accutase or Accumax (Innovative Cell Technologies, Inc.) for accurate counting.
2. Take 100 μL of cell suspension and transfer to another tube. Add 100 μL of prepared Trypan Blue (0.3 - 0.4%) solution to the tube. (This makes x 2 diluted solution).
3. Pipet the solution gently

Note Dilution rate should be optimized if cell numbers are high.

Counting
 

1) Take 10 μL of trypan blue stained cell suspension. Inject the solution from sample inlets slowly.
2) Count all cells on compartment ① - ④

• If the cells are on border lines, count those on 2 border lines only. (Red line, see Fig.2)
• Count Live cells (Unstained) and / or Dead cells (Stained)

3) Calculate cell numbers < Cell numbers in 1 mL >

• Live cells = ( All live cells / counted compartments) x dilution rate x 104
• Dead cells = ( All dead cells / counted compartments) x dilution rate x 104
• Viability (%) = Live cells / All cells (=Live cells + Dead cells) x 100

Note High speed injection may cause leak to other chambers.

Tips and Notes
 

• For accurate counting, adjust cell numbers to 100 – 500 cells / 1 mm2.
• Empty chamber may have small objects, but this does not affect to count.
• This is reference only. Detail counting method or for more information, please see experiment protocol book.

Success Stories
 

1. University of South Florida 
   • "They worked well and I liked the fact that you can read 4 samples at once."
2. Children's National Medical Center
   • "I liked them."