BioMasher III, 50 sets
BioMasher III, 50 sets
Cat. No. FNK-893131
Biomasher III
Procedures & Protocol
Research Articles
Generation of a polyclonal antibody against the CsPiezo and Western blot analysis
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Product Name |
Cat. No. |
Size |
Link |
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Biomasher III (Non-sterile) |
FNK-893131-Trial |
1 set |  |
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FNK-893131 |
50 sets |
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NIH NSN# 664000N173203
Description
Disposable Homogenizer. Non-sterilled, Autoclavable. It makes protein and nucleic acid extractions easier. Put samples into filter tube and insert pestle. Grinding samples by pestle by hand or motorized rotator named "Power Masher II". After that, centrifuge at 6,000 to 10,000 x g for 10 to 30 sec.
Biomasher products
Disposable Homogenizer BioMasher - The best way to homogenize your samples!
BioMasher - I
- Two types of pestle (with O-ring or without O-ring) are available. To avoid spill of liquid infectious samples, it is good for using "with O-ring" pestle.
- For soft tissue samples:
- Put samples into a filter cup and insert a pestle. Then centrifuge at 10,000 to 15,000 x g for 10 to 30 sec.
- For hard tissue samples:
- The tip of the pestle has " + " shape blade. This makes it possible to grind samples (which are difficult to be homogenized by only centrifuge) by pushing and twisting pestle.
BioMasher - II
- The combination of texture both pestle and inside of tube, homogenization efficiency is highly increased.
- Centrifuge is not required.
- Put samples into a tube and insert a pestle. Grinding samples by pestle by hand or motorized rotator named "Power Masher II".
- Please see the handling video here.
BioMasher - III
- Technology of BioMasher- I and II are combined.
- Put samples into a filter tube and insert a pestle. Grinding samples by pestle by hand or motorized rotator named "Power Masher II". After that, centrifuge at 6,000 to 10,000 x g for 10 to 30 sec.
- Please see the handling video from here.
BioMasher - IV
- Homogenizing samples by automated sample disrupter unit (i.e. FastPrep system or similar products) by using Zirconia beads.
- This system is suitable for bacteria, yeast and cultured cell samples.
Option
" Power Masher II"
This is a Pestle Motor for BioMasher II & III. It needs two (size) AA batteries (Accessories).
The Best Way for Homogenizing Your Samples
Disposable Homogenizer the "BioMasher"
The Best Way for Homogenizing Your Samples
BioMasher Series Comparison
Small to Large Size Tissues, Plants, Cell Pellets, Frozen Samples
- FAST TISSUE GRINDING to HOMOGENIZE
- DISPOSABLE & Premium Certified Plastics
- AUTOCLAVABLE & STERILIZED
- Multiple Types for various experimental designs
For more information about BioMasher products, please click "Go" button for BioMasher I - V and BioMasher SP
| # | BioMasher I | BioMasher II | BioMasher III | BioMasher V | BioMasher SP |
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| Shape |  |
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| Recovered Amount | Low | High | High | High | High |
| Heat Generation | No | Adjustable | Adjustable | Adjustable | Adjustable |
| Required Instrument | Centrifuge | None | Centrifuge | Centrifuge | None |
| Option types | 1.5 ml / 2.0 ml O-ring, PE/PP filter, 30/120 pieces(set) |
1.5 ml tube EOG-sterilized, Non-sterilized |
2.0 ml tube 50 pieces (set) |
15 mL tube (Large size) EOG-sterilized, Non-sterilized, 20 /100 pieces (set) |
15 mL tube (Large size) EOG-sterilized, Non-sterilized, 40 /100 pieces (set) |
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Appropriate Sample Type for BioMasher series
| Lab# | BioMasher I | BioMasher II | BioMasher III | BioMasher V | BioMasher SP |
|---|---|---|---|---|---|
| Soft Tissue | Excellent | Excellent | Excellent | Excellent | Excellent |
| Hard Tissue | Not good | Excellent | Excellent | Excellent | Excellent |
| Yeast | Not appropriate | Not appropriate | Not appropriate | Not appropriate | Not appropriate |
| Culture Cell | Not good | Not appropriate | Not good | Not good | Not appropriate |
| Product | Features | Procedure |
|---|---|---|
| BioMasher - I |
* Two types of pestle (with O-ring or without O-ring) are available. * For soft tissue samples: * For hard tissue samples: |
 |
| BioMasher - II |
* The combination of texture both pestle and inside of tube, homogenization efficiency is highly increased. * Centrifuge is not required. * Put samples into tube and insert pestle. Grinding samples by pestle by hand or motorized rotator named "Power Masher II". *Please see the handling video here |
 |
| BioMasher - III |
*Technology of BioMasher- I and II are combined. * Put samples into filter tube and insert pestle. Grinding samples by pestle by hand or motorized rotator named "Power Masher II". After that, centrifuge at 6,000 to 10,000 x g for 10 to 30 sec. *Please see the handling video from here. |
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| BioMasher - V |
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| BioMasher - SP |
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| Power Masher II |
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Comparison of RNA extraction efficiency among BioMasher I, II, and III
[Method]
RNA was extracted from various organs of a mouse (Crlj:CD1 [ICR]: Adult mouse of 8 weeks or older) using BioMasher I-III according to the attached protocol. As a control, RNA was extracted by using a pestle of SSI and a 1.5 mL tube of TreffLab.
[Results]
The results are summarized below.
| Tissue | RNA Extraction Ratio (RNA (μg)/Sample (mg)) | |||
| BioMasher I | BioMasher II | BioMasher III | Pestle/SSI & | |
| 1.5 mL tube/TreffLab | ||||
| Liver | 1 | 1.5 | 1.32 | 0.92 |
| Kidney | 0.26 | 1.87 | 4.66 | 0.15 |
| Cerebrum | 0.16 | 1.14 | - | 0.12 |
| Spleen | 0.15 | 5.53 | - | 0.36 |
| Heart | 0.08 | 1.02 | 0.5 | 0.12 |
| Tail | 0.03 | 0.43 | - | 0.16 |
| Muscle | 0.02 | 0.8 | 0.24 | 0.43 |
*Choose appropriate model of BioMasher for the maximum extraction ratio of RNA depending on tissue type.
References
- Tsuji M. et al., An immunostimulatory glycolipid that blocks SARS-CoV-2, RSV, and influenza infections in vivo. Nat Commun. 2023 Jul 5;14(1):3959.
- Ittiprasert W. et al., Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni. Cell Rep Methods. 2023 Jul 24;3(7):100535.
- Koo, J. W., Quintanilla-Dieck, L., Jiang, M., Liu, J., Urdang, Z. D., Allensworth, J. J., ... & Steyger, P. S. (2015). Endotoxemia-mediated inflammation potentiates aminoglycoside-induced ototoxicity. Science Translational Medicine, 7(298), 298ra118.
